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Product Info Summary
| SKU: | EK0562 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Mouse E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine®
SKU/Catalog Number
EK0562
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse E-Cadherin 1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cdh1 in cell culture supernatants, serum and plasma (heparin). . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0562)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: D157-V709
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0562 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0562 is reactive to Cdh1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK0562 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CDH1
E-Cadherin, also called Cadherin 1 (CDH1), Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca (2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0562-CAP | Anti-Mouse Cdh1 Pre-coated 96-well strip microplate | 1 |
| EK0562-ST | Mouse Cdh1 Standard | 2 vials, 10 ng/tube |
| EK0562-DA | Mouse Cdh1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.050 | 0.132 | 0.204 | 0.374 | 0.645 | 1.118 | 1.865 | 2.042 |
Data Example Images
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Mouse E-Cadherin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CDH1, Dilution ratio of 1:200, concentration in serum and plasma is 262-339 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 242 | 1644 | 5631 | 217 | 1771 | 5410 |
| Standard deviation | 14.52 | 118.36 | 360.38 | 14.53 | 146.99 | 394.93 |
| CV (%) | 6% | 7.2% | 6.4% | 6.7% | 8.3% | 7.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CDH1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 242 | 247 | 246 | 242 | 244 | 2.27 | 0.9% |
| Sample 2 | 1644 | 1884 | 1788 | 1601 | 1729 | 113.04 | 6.5% |
| Sample 3 | 5631 | 5520 | 5145 | 4973 | 5317 | 268.19 | 5% |
Protein Target Info & Infographic
Gene/Protein Information For Cdh1 (Source: Uniprot.Org, NCBI)
Gene Name
Cdh1
Full Name
Cadherin-1
Weight
98256 MW
Alternative Names
Cadherin-1;ARC-1;Epithelial cadherin;E-cadherin;Uvomorulin;CD324;E-Cad/CTF1;E-Cad/CTF2;E-Cad/CTF3;Cdh1; CDH1 Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO cadherin 1 cadherin-1|CAM 120/80|E-cadherin 1|cadherin 1, E-cadherin (epithelial)|cadherin 1, type 1, E-cadherin (epithelial)|calcium-dependent adhesion protein, epithelial|cell-CAM 120/80|epididymis secretory sperm binding protein|epithelial cadherin|uvomorulin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Cdh1, check out the Cdh1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cdh1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine® (EK0562)
Hello CJ!
EK0562 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Evaluation on biological compatibility of carboxymethyl chitosan as biomaterials for antitumor drug delivery:
Preparation and pharmacological evaluation of norcantharidin-conjugated carboxymethyl chitosan in mice bearing hepatocellular carcinoma
Experiment research of cisplatin implants inhibiting transplantation tumor growth and regulating the expression of KLK7 and E-cad of tumor-bearing mice with gastric cancer
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Mouse E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated CDH1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CDH1 antigen?
Verified Customer
Verified customer
Asked: 2019-12-08
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-12-08
Question
Q: What is the optimal O.D. value for CDH1 ELISA kit? I performed your CDH1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CDH1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CDH1 ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of CDH1 that can be declared/interpreted as positive by the above standard is 15pg/ml.
Boster Scientific Support
Answered: 2019-12-02
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
H. Jha
Verified customer
Asked: 2019-06-07
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-06-07
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
B. White
Verified customer
Asked: 2018-12-14
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-12-14
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
P. Wang
Verified customer
Asked: 2018-10-28
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-10-28
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CDH1 in Mouse plasma ? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-10-27
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CDH1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-10-27
Question
Q: how to analyze ELISA data? I have obtained CDH1 level in plasma .
C. Turner
Verified customer
Asked: 2018-03-06
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-03-06
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-06-25
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-06-25
