Product Info Summary
SKU: | M03905-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-CDC27 Monoclonal Antibody
SKU/Catalog Number
M03905-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CDC27 Monoclonal Antibody catalog # M03905-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDC27 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03905-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
5C12
Isotype
IgG
Immunogen
Purified recombinant fragment of human CDC27 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M03905-1 is reactive to CDC27 in Human
Reconstitution
Calculated molecular weight
91867 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03905-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of CDC27 using Anti-CDC27 Monoclonal Antibody (M03905-1).
Immunohistochemistry (IHC) analysis of paraffin-embedded Lung Cancer Tissues (left) and colon cancer tissues (right) with DAB staining using CDC27 Monoclonal Antibody.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-CDC27 Monoclonal Antibody M03905-1
Western Blot (WB) analysis using CDC27 Monoclonal Antibody against CDC27-hIgGFc transfected HEK293 cell.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CDC27 (Source: Uniprot.org, NCBI)
Gene Name
CDC27
Full Name
Cell division cycle protein 27 homolog
Weight
91867 MW
Superfamily
APC3/CDC27 family
Alternative Names
Cell division cycle protein 27 homolog;Anaphase-promoting complex subunit 3;APC3;CDC27 homolog;CDC27Hs;H-NUC;CDC27;ANAPC3, D0S1430E, D17S978E; CDC27 ANAPC3, APC3Hs, D0S1430E, D17S978E, H-NUC, HNUC, NUC2, CDC27 cell division cycle 27 cell division cycle protein 27 homolog|D0S1430E, D17S978E|anaphase promoting complex subunit 3|anaphase-promoting complex, protein 3|cell division cycle 27 homolog|nuc2 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC27, check out the CDC27 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDC27 Monoclonal Antibody (M03905-1)
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No publications found for M03905-1
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