Product Info Summary
SKU: | M00111 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Drosha Rabbit Monoclonal Antibody
SKU/Catalog Number
M00111
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Drosha Rabbit Monoclonal Antibody catalog # M00111. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Drosha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00111)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABEF-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Drosha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00111 is reactive to DROSHA in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
159 kDa
Calculated molecular weight
159316 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00111 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: human 293T whole cell, human Hela whole cell, human HepG2 whole cell, human K562 whole cell
IHC: human stomach cancer tissue, human stomach cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Drosha using anti-Drosha antibody (M00111).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Drosha antigen affinity purified monoclonal antibody (Catalog # M00111) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Drosha at approximately 159 kDa. The expected band size for Drosha is at 159 kDa.
Click image to see more details
Figure 2. IHC analysis of Drosha using anti-Drosha antibody (M00111).
Drosha was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Drosha Antibody (M00111) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Drosha using anti-Drosha antibody (M00111).
Drosha was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Drosha Antibody (M00111) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For DROSHA (Source: Uniprot.org, NCBI)
Gene Name
DROSHA
Full Name
Ribonuclease 3
Weight
159316 MW
Superfamily
ribonuclease III family
Alternative Names
Ribonuclease 3;3.1.26.3 ;Protein Drosha ;Ribonuclease III;RNase III;p241;DROSHA;RN3, RNASE3L, RNASEN; Drosha|1110013A17Rik, AI874853, Etoh, Etohi2, R, Rn3, Rnasen|drosha, ribonuclease type III|ribonuclease 3|RNase III|ethanol induced 2|protein Drosha|ribonuclease III, nuclear
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DROSHA, check out the DROSHA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DROSHA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Drosha Rabbit Monoclonal Antibody (M00111)
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3 Customer Q&As for Anti-Drosha Rabbit Monoclonal Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cerebellum using anti-Drosha Rabbit Monoclonal antibody M00111. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-20
Question
We are currently using anti-Drosha Rabbit Monoclonal antibody M00111 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-15
Answer
The anti-Drosha Rabbit Monoclonal antibody (M00111) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-15
Question
See below the WB image, lot number and protocol we used for cerebellum using anti-Drosha Rabbit Monoclonal antibody M00111. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-05-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-24