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Product Info Summary
| SKU: | A00111-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Ribonuclease 3 Drosha Antibody
SKU/Catalog Number
A00111-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Ribonuclease 3 Drosha Antibody catalog # A00111-2. Tested in WB,ICC,IF,IHC,Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ribonuclease 3 Drosha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00111-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00111-2 is reactive to DROSHA in Human
Reconstitution
Calculated molecular weight
159316 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00111-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of DROSHA using Anti-Ribonuclease 3 Drosha Antibody (A00111-2).
ICC staining Drosha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-Ribonuclease 3 Drosha Antibody A00111-2
Western blot analysis of Drosha on Hela cell and SiHa cell lysates using anti-Drosha antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For DROSHA (Source: Uniprot.org, NCBI)
Gene Name
DROSHA
Full Name
Ribonuclease 3
Weight
159316 MW
Superfamily
ribonuclease III family
Alternative Names
Ribonuclease 3;3.1.26.3 Drosha|1110013A17Rik, AI874853, Etoh, Etohi2, R, Rn3, Rnasen|drosha, ribonuclease type III|ribonuclease 3|RNase III|ethanol induced 2|protein Drosha|ribonuclease III, nuclear
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DROSHA, check out the DROSHA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DROSHA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ribonuclease 3 Drosha Antibody (A00111-2)
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