Ribonuclease 3 Antibodies

Ribonuclease 3 (Drosha)

Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Part of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to discharge precursor miRNA (pre-miRNA) from the nucleus.

Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing centre 11 bp from the dsRNA- ssRNA junction) to discharge hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to create mature miRNAs. Involved also in pre-rRNA processing.

Gene Information

Mouse
Gene Name:	Drosha
Uniprot:	Q5HZJ0
Entrez:	14000

Family

Belongs to:
ribonuclease III family

Alternative Names

drosha, double-stranded RNA-specific endoribonuclease; drosha, ribonuclease type III; EC 3.1.26.3; ETOHI2; nuclear RNase III Drosha; p241; Protein Drosha; putative protein p241 which interacts with transcription factor Sp1; putative ribonuclease III; RANSE3L; ribonuclease 3; Ribonuclease III; ribonuclease III, nuclear; RN3nuclear; RNase III

Molecular Weight

Mass (kDA):
158.828 kDA

Genomic Context

Mouse
Location:	15|15 A1
Sequence:	15;

Diseases

• Malignant Neoplasms
• Neoplasms
• Carcinoma
• Cell Transformation, Neoplastic
• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Ovarian Neoplasm
• Virus Diseases
• Digeorge Syndrome
• Malignant Paraganglionic Neoplasm
• Neoplasm Metastasis
• Malignant Neoplasm Of Ovary
• Carcinogenesis

• Tumor Progression
• Leukemia
• Amyotrophic Lateral Sclerosis
• Lung Neoplasms
• Epithelial Ovarian Cancer
• Adenocarcinoma
• Malignant Neoplasm Of Lung

Pathways

• Rna Interference
• Gene Silencing
• Translation
• Rna Processing
• Pathogenesis
• Cell Proliferation
• Localization
• Cell Cycle
• Cell Growth
• Cell Death
• Spermatogenesis
• Regulation Of Gene Expression
• Oncogenesis

• Pre-mirna Processing
• Cell Development
• Viral Replication
• Cell Differentiation
• Angiogenesis
• Stem Cell Maintenance
• Reverse Transcription

PTMs

• Cleavage
• Phosphorylation
• Methylation

• Reduction
• Hydroxylation
• Deacetylation

Research Areas

• Epigenetics

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Drosha

Best Uses Of The DROSHA Marker

There are many options available when you're looking for an anti-DROSHA marker. There are a variety of options to determine whether or absence of DROSHA. These include primary antibodies, ICC and ICC-Phorer. How do you know if your antibodies are specific? Read on to discover the benefits and applications of these options for antibodies.

Anti-DROSHA Marker

Boster Bio has a monoclonal antibody available that reacts with both human and mouse samples. It detects the DROSHA marker. Boster antibodies have been extensively validated on immunohistochemistry, Western Blotting, and ELISA, and can be stored at two to eight degrees Celsius. A package of 4mg Trehalose contains the boster anti-DROSHA antibody.

Immunofluorescence

DROSHA (also known as deoxyribonuclease) is an essential enzyme for pre-rRNA processing. It cuts the stem-loop pre-miRNA molecules and then releases the resulting hairpin-shaped molecules. DROSHA serves multiple purposes which include regulating the translation, processing and release of pre-miRNAs. It is also a crucial component of the microprocessor complex that is responsible for processing and releasing precursor miRNAs from the nucleus.

Primary antibodies

A range of primary antibodies can be generated by immunizing a host animal with antigens that are of importance and then extracting the antibody from its sera or eggs. This is known as manufacturing polyclonal antibodies. In this instance the primary antibodies were made with rabbit polyclonal Anti ALP or monoclonal Anti-runx2.

By combining primary and secondary antibodies, it is possible to create an antigen-specific dual-labeling system for specimens. Dual labeling allows researchers the chance to ask more questions about each specimen and also gain additional context information. Secondary antibodies are basically antibodies that can be mixed with different polymers. This means that the antibody can be utilized in conjunction with other reagents or antibodies, which allows for more effective analyses.

The DROSHA marker is versatile and can be used in a variety of experiments, such as the use of flow cytometry as well as immunofluorescence assays. The DROSHA marker is a commonly used marker to identify tumor-related proteins. Boster Bio Primary antibodies based on the DROSHA marker were extensively tested. Currently, several companies sell these primary antibodies. You can also buy them directly from the manufacturer.

Steven Boster's history

You have reached the right spot if you are looking for Steve Boster's past or current whereabouts. There's plenty of information on the internet about Steve Boster. This includes his current address, previous addresses family members, known addresses, as well as mobile phone numbers. You can also look up his age or state to limit your search. You can access his public records if want to know more information about Steve Boster.

Stephen Boster, 68, passed away on June 6, 2022. He was born on January 24, 1922 in Joliet, IL. He was a long-standing sales manager and served in the U.S. Army. He was an active member of Concordia Hall in Staunton, VA. His parents are also survived by his two daughters Natosha and Crystal Boster, 6 grandchildren, and 4 brothers and sisters, Jack and Sandra Blanton, and Lisa Milton. He is also survived by a number of nieces and nephews.