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Product Info Summary
| SKU: | M01698 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-DDR2 Monoclonal Antibody
SKU/Catalog Number
M01698
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DDR2 Monoclonal Antibody catalog # M01698. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDR2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01698)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACEB-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DDR2 This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01698 is reactive to DDR2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
v
Calculated molecular weight
210715 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01698 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: Jurkat cell, NIH/3T3 cell
Validation Images & Assay Conditions
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Western blot analysis of DDR2 expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For DDR2 (Source: Uniprot.org, NCBI)
Gene Name
DDR2
Full Name
Discoidin domain-containing receptor 2
Weight
210715 MW
Superfamily
protein kinase superfamily
Alternative Names
Myosin light chain kinase, smooth muscle;MLCK;smMLCK;2.7.11.18;Kinase-related protein;KRP;Telokin;Myosin light chain kinase, smooth muscle, deglutamylated form;MYLK;MLCK, MLCK1, MYLK1; DDR2 MIG20a, NTRKR3, TKT, TYRO10, WRCN discoidin domain receptor tyrosine kinase 2 discoidin domain-containing receptor 2|CD167 -like family member B|cell migration-inducing protein 20|discoidin domain receptor 2|discoidin domain receptor family, member 2|discoidin domain-containing receptor tyrosine kinase 2|hydroxyaryl-protein kinase|migration-inducing gene 16 protein|neurotrophic tyrosine kinase receptor related 3|receptor protein-tyrosine kinase TKT|tyrosine-protein kinase TYRO10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDR2, check out the DDR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDR2 Monoclonal Antibody (M01698)
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No publications found for M01698
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-DDR2 Monoclonal Antibody
Question
I see that the anti-DDR2 Monoclonal antibody M01698 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-08
Question
What sample have you tested on WB/IHC with M01698?
Verified customer
Asked: 2019-12-10
Answer
For the Anti-DDR2 Monoclonal Antibody M01698, Western Blot analysis of BIRC2 expression in HepG2 cell lysate.
Boster Scientific Support
Answered: 2019-12-15
Question
My boss were well pleased with the WB result of your anti-DDR2 Monoclonal antibody. However we have observed positive staining in skin cell membrane using this antibody. Is that expected? Could you tell me where is DDR2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
From what I have seen in literature, skin does express DDR2. Generally DDR2 expresses in cell membrane. Regarding which tissues have DDR2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Hair follicle dermal papilla, and Uterus, Pubmed ID: 14702039
Heart, and Thymus, Pubmed ID: 8247548
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-16
Question
Will anti-DDR2 Monoclonal antibody M01698 work for WB with hair follicle dermal papilla uterus?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
According to the expression profile of hair follicle dermal papilla uterus, DDR2 is highly expressed in hair follicle dermal papilla uterus. So, it is likely that anti-DDR2 Monoclonal antibody M01698 will work for WB with hair follicle dermal papilla uterus.
Boster Scientific Support
Answered: 2019-08-12
Question
Do you have a BSA free version of anti-DDR2 Monoclonal antibody M01698 available?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DDR2 Monoclonal antibody M01698 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-12
Question
Will M01698 anti-DDR2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
You can see on the product datasheet, M01698 anti-DDR2 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-27
Question
We are currently using anti-DDR2 Monoclonal antibody M01698 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
M. Collins
Verified customer
Asked: 2019-05-03
Answer
The anti-DDR2 Monoclonal antibody (M01698) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-03
Question
See attached the WB image, lot number and protocol we used for hair follicle dermal papilla uterus using anti-DDR2 Monoclonal antibody M01698. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-04-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-24
Question
My question regards to test anti-DDR2 Monoclonal antibody M01698 on human hair follicle dermal papilla uterus for research purposes, then I may be interested in using anti-DDR2 Monoclonal antibody M01698 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-02-13
Answer
The products we sell, including anti-DDR2 Monoclonal antibody M01698, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for hair follicle dermal papilla uterus using anti-DDR2 Monoclonal antibody M01698. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-05-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-17
Question
I was wanting to use your anti-DDR2 Monoclonal antibody for WB for human hair follicle dermal papilla uterus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human hair follicle dermal papilla uterus identification?
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
As indicated on the product datasheet, M01698 anti-DDR2 Monoclonal antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human hair follicle dermal papilla uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-21
Question
Is a blocking peptide available for product anti-DDR2 Monoclonal antibody (M01698)?
M. Rodriguez
Verified customer
Asked: 2018-02-07
Answer
We do provide the blocking peptide for product anti-DDR2 Monoclonal antibody (M01698). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-02-07
Question
My question regarding product M01698, anti-DDR2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Wu
Verified customer
Asked: 2016-02-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01698 anti-DDR2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-02-04
Question
We have seen staining in mouse heart thymus. Are there any suggestions? Is anti-DDR2 Monoclonal antibody supposed to stain heart thymus positively?
O. Collins
Verified customer
Asked: 2014-09-29
Answer
From literature heart thymus does express DDR2. From Uniprot.org, DDR2 is expressed in adipose tissue, heart thymus, hair follicle dermal papilla uterus, skin, cervix carcinoma, among other tissues. Regarding which tissues have DDR2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Hair follicle dermal papilla, and Uterus, Pubmed ID: 14702039
Heart, and Thymus, Pubmed ID: 8247548
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2014-09-29
Question
Is this M01698 anti-DDR2 Monoclonal antibody reactive to the isotypes of DDR2?
A. Zhang
Verified customer
Asked: 2014-09-18
Answer
The immunogen of M01698 anti-DDR2 Monoclonal antibody is A synthesized peptide derived from human DDR2 This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-09-18
