Anti-Cytochrome P450 8B1 CYP8B1 Antibody

Product Name

Anti-Cytochrome P450 8B1 CYP8B1 Antibody

View all CYP8B1 Antibodies

SKU/Catalog Number

A05518

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Cytochrome P450 8B1 CYP8B1 Antibody (Catalog # A05518). Tested in WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytochrome P450 8B1 CYP8B1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05518)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human Cytochrome P450 8B1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05518 is reactive to CYP8B1 in Human

Applications

A05518 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

111 kDa

Calculated molecular weight

58068 MW

Background of CYP8B1

Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids.

Gaafvels M., Genomics 56:184-196(1999).
Ota T., Nat. Genet. 36:40-45(2004).
Muzny D.M., Nature 440:1194-1198(2006)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

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Western blot analysis of extracts from 293 cells, using Cytochrome P450 8B1 antibody A05518.

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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using Cytochrome P450 8B1 antibody A05518.

Gene/Protein Information For CYP8B1 (Source: Uniprot.org, NCBI)

Alternative Names

7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase CYP8B1 CP8B, CYP12 cytochrome P450 family 8 subfamily B member 1 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase|7 alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase|CYPVIIIB1|cytochrome P450 8B1|cytochrome P450, family 8, subfamily B, polypeptide 1|cytochrome P450, subfamily VIIIB (sterol 12-alpha-hydroxylase), polypeptide 1|sterol 12-alpha-hydroxylase

For more info on CYP8B1, check out the CYP8B1 Infographic

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In this infographic, you will see the following information for CYP8B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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