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Product Info Summary
| SKU: | M10244 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Macaque monkey |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-Myeloid-Associated Differentiation Marker (MYADM) Monoclonal Antibody
View all Myeloid-Associated Differentiation Marker Antibodies
SKU/Catalog Number
M10244
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Myeloid-Associated Differentiation Marker (MYADM) Monoclonal Antibody (Catalog # M10244). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Macaque Monkey.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Myeloid-Associated Differentiation Marker (MYADM) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M10244)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: MYADM/972
Isotype
IgG1, kappa
Immunogen
Recombinant human MYADM protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M10244 is reactive to Myadm in Human, Macaque monkey
Applications
M10244 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
35274 MW
Background of Myeloid-Associated Differentiation Marker
Recognizes a myeloid associated differentiation antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of human and monkey myeloid cells. This monoclonal antibody is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-MYDAM Monoclonal Antibody (MYADM/972).
Protein Target Info & Infographic
Gene/Protein Information For Myadm (Source: Uniprot.org, NCBI)
Gene Name
Myadm
Full Name
Myeloid-associated differentiation marker
Weight
35274 MW
Superfamily
MAL family
Alternative Names
MYADM ; Myeloid Upregulated Protein; Protein SB135; SB135 Myadm|D7Wsu62, D7Wsu62e|myeloid-associated differentiation marker|myeloid-associated differentiation marker|myeloid up-regulated protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Myadm, check out the Myadm Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Myadm: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myeloid-Associated Differentiation Marker (MYADM) Monoclonal Antibody (M10244)
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No publications found for M10244
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Myeloid-Associated Differentiation Marker (MYADM) Monoclonal Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Myeloid-Associated Differentiation Marker (MYADM) Monoclonal antibody M10244. Let me know if you need anything else.
M. Rodriguez
Verified customer
Asked: 2020-02-21
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-21
Question
We are currently using anti-Myeloid-Associated Differentiation Marker (MYADM) Monoclonal antibody M10244 for monkey tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, monkey. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
The anti-Myeloid-Associated Differentiation Marker (MYADM) Monoclonal antibody (M10244) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-25
Question
Will anti-Myeloid-Associated Differentiation Marker (MYADM) Monoclonal antibody M10244 work for IHC with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
According to the expression profile of cervix carcinoma, MYADM is highly expressed in cervix carcinoma. So, it is likely that anti-Myeloid-Associated Differentiation Marker (MYADM) Monoclonal antibody M10244 will work for IHC with cervix carcinoma.
Boster Scientific Support
Answered: 2019-07-30
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-Myeloid-Associated Differentiation Marker (MYADM) Monoclonal antibody M10244. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-20
Question
Does M10244 anti-Myeloid-Associated Differentiation Marker (MYADM) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-29
Answer
It shows on the product datasheet, M10244 anti-Myeloid-Associated Differentiation Marker (MYADM) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-29
Question
Is a blocking peptide available for product anti-Myeloid-Associated Differentiation Marker (MYADM) Monoclonal antibody (M10244)?
Verified Customer
Verified customer
Asked: 2017-09-25
Answer
We do provide the blocking peptide for product anti-Myeloid-Associated Differentiation Marker (MYADM) Monoclonal antibody (M10244). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-09-25
Question
I see that the anti-Myeloid-Associated Differentiation Marker (MYADM) Monoclonal antibody M10244 works with IHC, what is the protocol used to produce the result images on the product page?
B. Jackson
Verified customer
Asked: 2014-10-20
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-10-20
