Anti-Mre11 Antibody

Product Name

Anti-Mre11 Antibody

View all Mre11 Antibodies

SKU/Catalog Number

A00731

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Mre11 Antibody (Catalog # A00731). Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Mre11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00731)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 68-706 of mouse Mre11 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00731 is reactive to MRE11A in Mouse

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

80593 MW

Background of Mre11

Mre11 is a component of the MRN complex (Mre11/Rad50/Nbs1), which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them close. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres, the MRN complex may modulate t-loop formulation. Anti-Mre11 Antibody is useful for researcher interested in DNA Damage and Repair and Nuclear Signaling Research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00731 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For MRE11A (Source: Uniprot.org, NCBI)

Alternative Names

Mre11, Double-strand break repair protein MRE11a, DNA recombination and repair protein, Meiotic recombination 11 homolog A, Mre11a

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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