Anti-Phospho-CREB S133 CREB1 Antibody

Product Name

Anti-Phospho-CREB S133 CREB1 Antibody

View all CREB Antibodies

SKU/Catalog Number

P00577

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Phospho-CREB S133 CREB1 Antibody (Catalog # P00577). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Phospho-CREB S133 CREB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00577)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

CREB phospho peptide corresponding to amino acid residues 122-147 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00577 is reactive to CREB1 in Human, Mouse, Rat

Applications

P00577 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

36688 MW

Background of CREB

The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMP-dependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II. Alternate splicing of this gene results in two transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:1,000 - 1:6,000
IHC: 20 µg/ml
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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CREB was detected in paraffin-embedded sections of tonsillar lymphocytes tissue using rabbit anti-CREB pS133 Antigen Affinity purified polyclonal antibody (Catalog # P00577) at 20 ug/mL. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

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Western blot analysis of phosphorylated CREB expression using rabbit anti-CREB pS133 Antigen Affinity purified polyclonal antibody (Catalog # P00577) at 1:500. Recombinant His-tagged human CREB was produced in E.coli and purified by metal affinity chromatography. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. Western blot of control (-) and in-vitro phosphorylated CREB (+) was used to show that the antibody reacts specifically with the phosphorylated form. Personal Communication, R. Screaton, The Salk Institute for Biological Studies.

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Western blot analysis of phosphorylated CREB expression using rabbit anti-CREB pS133 Antigen Affinity purified polyclonal antibody (Catalog # P00577) at 1:500. Recombinant His-tagged human CREB was produced in E.coli and purified by metal affinity chromatography. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. western blot of control (-) and in-vitro phosphorylated CREB (+) was used to show that the antibody reacts specifically with the phosphorylated form. Personal Communication, Boss, J., Emory University School of Medicine, Atlanta, GA.

Gene/Protein Information For CREB1 (Source: Uniprot.org, NCBI)

Alternative Names

active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB1; CREB-1; cyclic AMP-responsive element-binding protein 1; MGC9284; pCREB; transactivator protein CREB1 CREB, CREB-1 cAMP responsive element binding protein 1 cyclic AMP-responsive element-binding protein 1|active transcription factor CREB|cAMP-response element-binding protein-1|cyclic adenosine 3,5-monophosphate response element binding protein|cyclic adenosine 3,5-monophosphate response element-binding protein CREB|transactivator protein

For more info on CREB1, check out the CREB1 Infographic

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In this infographic, you will see the following information for CREB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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