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Product Info Summary
| SKU: | P00577 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Phospho-CREB S133 CREB1 Antibody
SKU/Catalog Number
P00577
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Phospho-CREB S133 CREB1 Antibody (Catalog # P00577). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Phospho-CREB S133 CREB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00577)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
CREB phospho peptide corresponding to amino acid residues 122-147 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00577 is reactive to CREB1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
36688 MW
Background of CREB
The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMP-dependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00577 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:1,000 - 1:6,000
IHC: 20 µg/ml
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
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CREB was detected in paraffin-embedded sections of tonsillar lymphocytes tissue using rabbit anti-CREB pS133 Antigen Affinity purified polyclonal antibody (Catalog # P00577) at 20 ug/mL. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
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Western blot analysis of phosphorylated CREB expression using rabbit anti-CREB pS133 Antigen Affinity purified polyclonal antibody (Catalog # P00577) at 1:500. Recombinant His-tagged human CREB was produced in E.coli and purified by metal affinity chromatography. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. Western blot of control (-) and in-vitro phosphorylated CREB (+) was used to show that the antibody reacts specifically with the phosphorylated form. Personal Communication, R. Screaton, The Salk Institute for Biological Studies.
Click image to see more details
Western blot analysis of phosphorylated CREB expression using rabbit anti-CREB pS133 Antigen Affinity purified polyclonal antibody (Catalog # P00577) at 1:500. Recombinant His-tagged human CREB was produced in E.coli and purified by metal affinity chromatography. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. western blot of control (-) and in-vitro phosphorylated CREB (+) was used to show that the antibody reacts specifically with the phosphorylated form. Personal Communication, Boss, J., Emory University School of Medicine, Atlanta, GA.
Protein Target Info & Infographic
Gene/Protein Information For CREB1 (Source: Uniprot.org, NCBI)
Gene Name
CREB1
Full Name
Cyclic AMP-responsive element-binding protein 1
Weight
36688 MW
Superfamily
bZIP family
Alternative Names
CREB pS133, Cyclic AMP-responsive element-binding protein 1, Active transcription factor CREB, cAMP responsive element binding protein 1, CREB 1, CREB-1, CREB1, MGC9284, Transactivator protein CREB1 CREB, CREB-1 cAMP responsive element binding protein 1 cyclic AMP-responsive element-binding protein 1|active transcription factor CREB|cAMP-response element-binding protein-1|cyclic adenosine 3,5-monophosphate response element binding protein|cyclic adenosine 3,5-monophosphate response element-binding protein CREB|transactivator protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CREB1, check out the CREB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CREB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-CREB S133 CREB1 Antibody (P00577)
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Customer Q&As
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16 Customer Q&As for Anti-Phospho-CREB S133 CREB1 Antibody
Question
We bought anti-Phospho-CREB S133 antibody for IHC on eye a few months ago. I am using mouse, and We want to use the antibody for WB next. My question regards examining eye as well as cervix carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
I have checked the website and datasheets of our anti-Phospho-CREB S133 antibody and it seems that P00577 has been validated on mouse in both IHC and WB. Thus P00577 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-30
Question
Do you have a BSA free version of anti-Phospho-CREB S133 antibody P00577 available?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-CREB S133 antibody P00577 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-20
Question
My boss were satisfied with the WB result of your anti-Phospho-CREB S133 antibody. However we have seen positive staining in leukemic t-cell nucleus using this antibody. Is that expected? Could you tell me where is CREB1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
From literature, leukemic t-cell does express CREB1. Generally CREB1 expresses in nucleus. Regarding which tissues have CREB1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 15579595
Boster Scientific Support
Answered: 2019-09-27
Question
Does P00577 anti-Phospho-CREB S133 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
You can see on the product datasheet, P00577 anti-Phospho-CREB S133 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-27
Question
Is a blocking peptide available for product anti-Phospho-CREB S133 antibody (P00577)?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
We do provide the blocking peptide for product anti-Phospho-CREB S133 antibody (P00577). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-02
Question
I was wanting to use your anti-Phospho-CREB S133 antibody for WB for rat eye on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat eye identification?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
You can see on the product datasheet, P00577 anti-Phospho-CREB S133 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat eye in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-20
Question
We are interested in using your anti-Phospho-CREB S133 antibody for positive regulation of rna polymerase ii transcriptional preinitiation complex assembly studies. Has this antibody been tested with western blotting on tonsillar lymphocytes tissue? We would like to see some validation images before ordering.
A. Parker
Verified customer
Asked: 2019-05-27
Answer
Thanks for your inquiry. This P00577 anti-Phospho-CREB S133 antibody is tested on tonsillar lymphocytes tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-27
Question
Will anti-Phospho-CREB S133 antibody P00577 work for WB with eye?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
According to the expression profile of eye, CREB1 is highly expressed in eye. So, it is likely that anti-Phospho-CREB S133 antibody P00577 will work for WB with eye.
Boster Scientific Support
Answered: 2019-05-16
Question
We are currently using anti-Phospho-CREB S133 antibody P00577 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
The anti-Phospho-CREB S133 antibody (P00577) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-14
Question
See below the WB image, lot number and protocol we used for eye using anti-Phospho-CREB S133 antibody P00577. Please let me know if you require anything else.
A. Krishna
Verified customer
Asked: 2018-12-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-Phospho-CREB S133 antibody P00577. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-17
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-17
Question
Can you help my question with product P00577, anti-Phospho-CREB S133 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-11
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00577 anti-Phospho-CREB S133 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-11
Question
We have seen staining in human testis. What should we do? Is anti-Phospho-CREB S133 antibody supposed to stain testis positively?
Verified Customer
Verified customer
Asked: 2017-07-31
Answer
From literature testis does express CREB1. From Uniprot.org, CREB1 is expressed in lung, testis, eye, embryonic kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have CREB1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 15579595
Boster Scientific Support
Answered: 2017-07-31
Question
Is this P00577 anti-Phospho-CREB S133 antibody reactive to the isotypes of CREB1?
N. Miller
Verified customer
Asked: 2017-05-04
Answer
The immunogen of P00577 anti-Phospho-CREB S133 antibody is CREB phospho peptide corresponding to amino acid residues 122-147 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-05-04
Question
I would like to test anti-Phospho-CREB S133 antibody P00577 on rat eye for research purposes, then I may be interested in using anti-Phospho-CREB S133 antibody P00577 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Roberts
Verified customer
Asked: 2014-01-21
Answer
The products we sell, including anti-Phospho-CREB S133 antibody P00577, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-01-21
Question
I see that the anti-Phospho-CREB S133 antibody P00577 works with WB, what is the protocol used to produce the result images on the product page?
Z. Anderson
Verified customer
Asked: 2013-01-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-01-28
