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Product Info Summary
| SKU: | M04245 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, Human, Mouse, Pig, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Coronin 1a Antibody
View all Coronin-1a Antibodies
SKU/Catalog Number
M04245
Size
100ul
Description
Boster Bio Anti-Coronin 1a Antibody catalog # M04245. Tested in IF, ICC, WB applications. This antibody reacts with Bovine, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Coronin 1a Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04245)
Host
Rabbit
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Polyclonal
Immunogen
C-terminal peptide of human coronin 1a couple to KLH
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04245 is reactive to CORO1A in Bovine, Human, Mouse, Pig, Rat
Reconstitution
Calculated molecular weight
51026 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04245 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:5,000, IF/ICC: 1:500-1:1,000
Validation Images & Assay Conditions
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Western blot analysis of tissue lysates using rabbit pAb to coronin 1a, M04245, dilution 1:5,000: [1] protein standard, [2] mouse brain, [3] rat brain, [4] cow cerebellum, [5] cow cortex, and [6] pig spinal cord. The strong single band about 55kDa corresponds to the coronin 1a protein.
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Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to coronin 1a, M04245, dilution 1:1,000 in red, and costained with mouse mAb to GFAP, dilution 1:1,000 in green. The blue is Hoechst staining of nuclear DNA. The coronin 1a antibody labels protein expressed in the cytoplasm of microglia cells, while GFAP antibody stains intermediate filaments in astrocytic cells.
Protein Target Info & Infographic
Gene/Protein Information For CORO1A (Source: Uniprot.org, NCBI)
Gene Name
CORO1A
Full Name
Coronin-1A
Weight
51026 MW
Superfamily
WD repeat coronin family
Alternative Names
Coronin-1A;Coronin-like protein A;Clipin-A;Coronin-like protein p57;Tryptophan aspartate-containing coat protein;TACO;CORO1A;CORO1; CORO1A CLABP, CLIPINA, HCORO1, IMD8, TACO, p57 coronin 1A coronin-1A|clipin-A|coronin, actin binding protein, 1A|coronin-1|coronin-like protein A|coronin-like protein p57|tryptophan aspartate-containing coat protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CORO1A, check out the CORO1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CORO1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Coronin 1a Antibody (M04245)
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No publications found for M04245
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Customer Q&As
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6 Customer Q&As for Anti-Coronin 1a Antibody
Question
Would anti-Coronin 1a antibody M04245 work for WB with kidney?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
According to the expression profile of kidney, CORO1A is highly expressed in kidney. So, it is likely that anti-Coronin 1a antibody M04245 will work for WB with kidney.
Boster Scientific Support
Answered: 2020-04-24
Question
Does anti-Coronin 1a antibody M04245 work on horse WB with kidney?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
Our lab technicians have not validated anti-Coronin 1a antibody M04245 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Coronin 1a antibody M04245 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-12
Question
We are currently using anti-Coronin 1a antibody M04245 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says bovine, human, mouse, pig, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
The anti-Coronin 1a antibody (M04245) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-25
Question
Our lab want to know about to test anti-Coronin 1a antibody M04245 on bovine kidney for research purposes, then I may be interested in using anti-Coronin 1a antibody M04245 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Krishna
Verified customer
Asked: 2018-03-27
Answer
The products we sell, including anti-Coronin 1a antibody M04245, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-27
Question
Is there a BSA free version of anti-Coronin 1a antibody M04245 available?
S. Anderson
Verified customer
Asked: 2016-09-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Coronin 1a antibody M04245 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-09-15
Question
See attached the WB image, lot number and protocol we used for kidney using anti-Coronin 1a antibody M04245. Please let me know if you require anything else.
E. Mangal
Verified customer
Asked: 2015-12-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-29
