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Product Info Summary
| SKU: | A00164-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IL17C Antibody Picoband®
SKU/Catalog Number
A00164-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL17C Antibody Picoband® catalog # A00164-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL17C Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00164-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human IL17C recombinant protein (Position: H19-V197).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00164-2 is reactive to IL17C in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa
Calculated molecular weight
21.765kDa
Background of IL-17C
IL17C, also known as CX2, is a protein that in humans is encoded by the IL17C gene. IL17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00164-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: mouse spleen tissue, mouse thymus tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL17C using anti-IL17C antibody (A00164-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17C antigen affinity purified polyclonal antibody (Catalog # A00164-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17C at approximately 29KD. The expected band size for IL17C is at 22KD.
Protein Target Info & Infographic
Gene/Protein Information For IL17C (Source: Uniprot.org, NCBI)
Gene Name
IL17C
Full Name
Interleukin-17C
Weight
21.765kDa
Superfamily
IL-17 family
Alternative Names
Interleukin-17C; IL-17C; Cytokine CX2; IL17C; UNQ561/PRO1122 IL17C CX2, IL-17C interleukin 17C interleukin-17C|cytokine CX2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL17C, check out the IL17C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL17C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL17C Antibody Picoband® (A00164-2)
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Customer Q&As
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4 Customer Q&As for Anti-IL17C Antibody Picoband®
Question
I would like to test anti-IL17C antibody A00164-2 on human lung for research purposes, then I may be interested in using anti-IL17C antibody A00164-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The products we sell, including anti-IL17C antibody A00164-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-27
Question
Does anti-IL17C antibody A00164-2 work on horse ELISA with quadriceps femoris?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
Our lab technicians have not tested anti-IL17C antibody A00164-2 on horse. You can run a BLAST between horse and the immunogen sequence of anti-IL17C antibody A00164-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse quadriceps femoris in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-30
Question
Is this A00164-2 anti-IL17C antibody reactive to the isotypes of IL17C?
N. Lewis
Verified customer
Asked: 2014-07-09
Answer
The immunogen of A00164-2 anti-IL17C antibody is E. coli-derived human IL17C recombinant protein (Position: H19-V197). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-07-09
Question
Will anti-IL17C antibody A00164-2 work for WB with lung?
H. Johnson
Verified customer
Asked: 2013-01-18
Answer
According to the expression profile of lung, IL17C is highly expressed in lung. So, it is likely that anti-IL17C antibody A00164-2 will work for WB with lung.
Boster Scientific Support
Answered: 2013-01-18
