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Product Info Summary
| SKU: | EK0789 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human IL-17C ELISA Kit PicoKine®
SKU/Catalog Number
EK0789
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-17C ELISA Kit PicoKine® (96 Tests). Quantitate Human IL17C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-17C ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0789)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: H19-V197
Sensitivity
<10 pg/ml
Assay Range
125 pg/ml - 8,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0789 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0789 is reactive to IL17C in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0789 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-17C
IL17C, also known as CX2, is a protein that in humans is encoded by the IL17C gene. IL17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0789-CAP | Anti-Human IL17C Pre-coated 96-well strip microplate | 1 |
| EK0789-ST | Human IL17C Standard | 2 vials, 10 ng/tube |
| EK0789-DA | Human IL17C Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
| O.D. | 0.112 | 0.209 | 0.270 | 0.396 | 0.653 | 0.970 | 1.422 | 1.821 |
Data Example Images
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Human IL-17C PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-17C, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 125 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-17C ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 188 | 1000 | 5861 | 172 | 1082 | 6289 |
| Standard deviation | 12.4 | 65 | 304.77 | 11.52 | 88.72 | 377.34 |
| CV (%) | 6.6% | 6.5% | 5.2% | 6.7% | 8.2% | 6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-17C in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 188 | 158 | 157 | 172 | 168 | 12.59 | 7.4% |
| Sample 2 | 1000 | 1030 | 955 | 941 | 981 | 35.48 | 3.6% |
| Sample 3 | 5861 | 6256 | 6014 | 5655 | 5946 | 219.44 | 3.6% |
Protein Target Info & Infographic
Gene/Protein Information For IL17C (Source: Uniprot.Org, NCBI)
Gene Name
IL17C
Full Name
Interleukin-17C
Weight
21765 MW
Superfamily
IL-17 family
Alternative Names
Interleukin-17C;IL-17C;Cytokine CX2;IL17C;UNQ561/PRO1122; IL17C CX2, IL-17C interleukin 17C interleukin-17C|cytokine CX2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL17C, check out the IL17C Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL17C: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IL-17C ELISA Kit PicoKine® (EK0789)
Hello CJ!
EK0789 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
‘Psoriasis 1’ reduces psoriasis‑like skin inflammation by inhibiting the VDR‑mediated nuclear NF‑κB and STAT signaling pathways
Psoriasis 1'reduces psoriasis%u2011like skin inflammation by inhibiting the VDR%u2011mediated nuclear NF%u2011%u03BAB and STAT signaling pathways
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human IL-17C ELISA Kit PicoKine®
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-08-04
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-08-04
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of IL-17C in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-17C ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-04-24
Question
Q: which procedure should I follow in order to thaw whole blood sample for IL-17C ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-17C for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-06-20
Question
Can EK0789 and EK0793 be used with skin biopsies? If yes, is there a need to modify the protocol?
Verified customer
Asked: 2019-03-27
Answer
Our lab has not worked on skin biopsies using the Human IL-17C ELISA Kit PicoKine™ (EK0789) and Human IL-17E/IL-25 ELISA Kit PicoKine™ (EK0793). We suggest you to run a pilot test using the kits. The following is the suggested sample preparation protocol; 1. Mince skin biopsies and then rinse skin biopsies in ice-cold PBS. 2. Place minced skin biopsies in tissue homogenizer. 3. Add ice-cold PBS. For a 0.5g piece of tissue, add 5ml of ice-cold PBS (Volumes of PBS must be determined in relation to the amount of skin biopsies present.) 5. Homogenize for several minutes at high speed. 6. Sonicate until no skin biopsies chunks remain. 7. Centrifuge at ~10000 x g for 10 minutes. The centrifugation force and time can be varied depending on the sample type. 8. Aspirate supernatant. 9. Aliquot supernatant and store at -20°C. There is no need to adjust the main protocol.
Boster Scientific Support
Answered: 2019-03-28
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-01-15
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-01-15
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
G. Babu
Verified customer
Asked: 2018-11-11
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-11-11
Question
Q: if the enzyme conjugated IL-17C antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-17C antigen?
Verified Customer
Verified customer
Asked: 2017-02-25
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-02-25
