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Product Info Summary
| SKU: | EK0790 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse IL-17C ELISA Kit PicoKine®
SKU/Catalog Number
EK0790
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-17C ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il17c in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IL-17C ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0790)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: D17-Q194
Sensitivity
<10 pg/ml
Assay Range
125 pg/ml - 8,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0790 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0790 is reactive to Il17c in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0790 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-17C
IL17C, also known as CX2, is a protein that in humans is encoded by the IL17C gene. IL17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0790-CAP | Anti-Mouse Il17c Pre-coated 96-well strip microplate | 1 |
| EK0790-ST | Mouse Il17c Standard | 2 vials, 10 ng/tube |
| EK0790-DA | Mouse Il17c Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
| O.D. | 0.106 | 0.162 | 0.221 | 0.339 | 0.550 | 0.851 | 1.310 | 2.039 |
Data Example Images
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Mouse IL-17C PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-17C, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 125 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-17C ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 150 | 1098 | 6281 | 140 | 1153 | 5857 |
| Standard deviation | 11.85 | 68.07 | 414.54 | 13.58 | 85.32 | 447.63 |
| CV (%) | 7.9% | 6.2% | 6.6% | 9.7% | 7.4% | 7.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-17C in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 150 | 157 | 168 | 157 | 158 | 6.44 | 4% |
| Sample 2 | 1098 | 1141 | 972 | 1172 | 1095 | 76.12 | 6.9% |
| Sample 3 | 6281 | 5461 | 5326 | 5304 | 5593 | 401.73 | 7.1% |
Protein Target Info & Infographic
Gene/Protein Information For Il17c (Source: Uniprot.Org, NCBI)
Gene Name
Il17c
Full Name
Interleukin-17C
Weight
21592 MW
Superfamily
IL-17 family
Alternative Names
Interleukin-17C;Il-17c;Cytokine CX2;Il17c; IL17C CX2, IL-17C interleukin 17C interleukin-17C|cytokine CX2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Il17c, check out the Il17c Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il17c: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse IL-17C ELISA Kit PicoKine® (EK0790)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Mouse IL-17C ELISA Kit PicoKine®
Question
Q: Can IL-17C ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
J. Phillips
Verified customer
Asked: 2020-01-07
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-01-07
Question
Q: What is the optimal O.D. value for IL-17C ELISA kit? I used your IL-17C ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL-17C even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-17C ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of IL-17C that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-10-18
Question
Q: which procedure should I follow in order to thaw whole blood sample for IL-17C ELISA after freezing?
E. Brown
Verified customer
Asked: 2019-09-16
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-17C for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-09-16
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL-17C using the EK0790 Mouse IL-17C Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-03-14
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2019-03-14
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-10-07
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-10-07
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
K. Jones
Verified customer
Asked: 2018-06-06
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-06-06
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
M. Surve
Verified customer
Asked: 2016-01-08
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-01-08
