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Product Info Summary
| SKU: | M12717 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-COPS7B Antibody (N-Term)
SKU/Catalog Number
M12717
Size
50 µl
Description
Boster Bio Anti-COPS7B Antibody (N-Term) (Catalog # M12717). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-COPS7B Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12717)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This COPS7B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 61-95 amino acids from human COPS7B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M12717 is reactive to COPS7B in Human
Reconstitution
Calculated molecular weight
29622 Da
Background of CSN7b
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M12717 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-COPS7B Antibody (N-Term) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HL-60 whole cell lysate
Lane 3: HT-29 whole cell lysate
Lane 4: Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 30 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M12717 staining COPS7B in human kidney tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Overlay histogram showing Hela cells stained with M12717 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M12717, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For COPS7B (Source: Uniprot.org, NCBI)
Gene Name
COPS7B
Full Name
COP9 signalosome complex subunit 7b
Weight
29622 Da
Superfamily
CSN7/EIF3M family
Alternative Names
COP9 signalosome complex subunit 7b, SGN7b, Signalosome subunit 7b, JAB1-containing signalosome subunit 7b, COPS7B, CSN7B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COPS7B, check out the COPS7B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COPS7B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-COPS7B Antibody (N-Term) (M12717)
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