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Product Info Summary
| SKU: | A04827 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GluR2/3 GRM3 Antibody
SKU/Catalog Number
A04827
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-GluR2/3 GRM3 Antibody (Catalog # A04827). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GluR2/3 GRM3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04827)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human GluR2/3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04827 is reactive to GRM3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
98879 MW
Background of mGluR3
G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.
Akiko Momiyama, J. Physiol., May 2003; 549: 75 - 92.
Mako Narisawa-Saito, PNAS, Mar 1999; 96: 2461.
Karolina Wosik, Brain, Dec 2004; 127: 2636 - 2648.
Chong-Hyun Kim, PNAS, Sep 2001; 98: 11725.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04827 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from mouse brain, using GluR2/3 antibody A04827.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using GluR2/3 antibody A04827.
Protein Target Info & Infographic
Gene/Protein Information For GRM3 (Source: Uniprot.org, NCBI)
Gene Name
GRM3
Full Name
Metabotropic glutamate receptor 3
Weight
98879 MW
Superfamily
G-protein coupled receptor 3 family
Alternative Names
Metabotropic glutamate receptor 3;mGluR3;GRM3;GPRC1C, MGLUR3; GRM3 GLUR3, GPRC1C, MGLUR3, mGlu3 glutamate metabotropic receptor 3 metabotropic glutamate receptor 3|glutamate receptor, metabotropic 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GRM3, check out the GRM3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GRM3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GluR2/3 GRM3 Antibody (A04827)
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No publications found for A04827
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-GluR2/3 GRM3 Antibody
Question
I have attached the WB image, lot number and protocol we used for caudate nucleus using anti-GluR2/3 antibody A04827. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-16
Question
I see that the anti-GluR2/3 antibody A04827 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-11
Question
Would anti-GluR2/3 antibody A04827 work for IHC with caudate nucleus?
Verified Customer
Verified customer
Asked: 2019-04-09
Answer
According to the expression profile of caudate nucleus, GRM3 is highly expressed in caudate nucleus. So, it is likely that anti-GluR2/3 antibody A04827 will work for IHC with caudate nucleus.
Boster Scientific Support
Answered: 2019-04-09
Question
Will anti-GluR2/3 antibody A04827 work on monkey WB with caudate nucleus?
Verified Customer
Verified customer
Asked: 2018-11-09
Answer
Our lab technicians have not validated anti-GluR2/3 antibody A04827 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-GluR2/3 antibody A04827 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey caudate nucleus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-09
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for caudate nucleus using anti-GluR2/3 antibody A04827. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-02-15
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-15
Question
Do you have a BSA free version of anti-GluR2/3 antibody A04827 available?
Verified Customer
Verified customer
Asked: 2018-02-02
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-GluR2/3 antibody A04827 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-02
Question
Is a blocking peptide available for product anti-GluR2/3 antibody (A04827)?
O. Edwards
Verified customer
Asked: 2017-10-10
Answer
We do provide the blocking peptide for product anti-GluR2/3 antibody (A04827). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-10
Question
Is this A04827 anti-GluR2/3 antibody reactive to the isotypes of GRM3?
Verified Customer
Verified customer
Asked: 2017-06-06
Answer
The immunogen of A04827 anti-GluR2/3 antibody is Synthesized peptide derived from human GluR2/3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-06
Question
Does A04827 anti-GluR2/3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Lewis
Verified customer
Asked: 2016-08-31
Answer
It shows on the product datasheet, A04827 anti-GluR2/3 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-08-31
Question
I was wanting to use your anti-GluR2/3 antibody for IHC for rat caudate nucleus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat caudate nucleus identification?
J. Taylor
Verified customer
Asked: 2015-08-19
Answer
As indicated on the product datasheet, A04827 anti-GluR2/3 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat caudate nucleus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-08-19
Question
My question regards to test anti-GluR2/3 antibody A04827 on rat caudate nucleus for research purposes, then I may be interested in using anti-GluR2/3 antibody A04827 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Collins
Verified customer
Asked: 2013-07-02
Answer
The products we sell, including anti-GluR2/3 antibody A04827, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-07-02
Question
We are currently using anti-GluR2/3 antibody A04827 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
M. Moore
Verified customer
Asked: 2013-03-12
Answer
The anti-GluR2/3 antibody (A04827) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-12
Question
Can you help my question with product A04827, anti-GluR2/3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Banerjee
Verified customer
Asked: 2013-01-09
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04827 anti-GluR2/3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-01-09
