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Product Info Summary
| SKU: | A04538-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Contactin 1/CNTN1 Antibody Picoband®
View all Contactin-1 Antibodies
SKU/Catalog Number
A04538-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Contactin 1/CNTN1 Antibody Picoband® catalog # A04538-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Contactin 1/CNTN1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04538-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Contactin 1/CNTN1 recombinant protein (Position: K225-H979).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04538-2 is reactive to CNTN1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
135 kDa
Calculated molecular weight
113.32kDa
Background of Contactin-1
Contactin 1, also known as CNTN1, is a protein which in humans is encoded by the CNTN1 gene. The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04538-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat brain tissue, mouse brain tissue
ICC/IF: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Contactin 1/CNTN1 using anti-Contactin 1/CNTN1 antibody (A04538-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Contactin 1/CNTN1 antigen affinity purified polyclonal antibody (Catalog # A04538-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Contactin 1/CNTN1 at approximately 135 kDa. The expected band size for Contactin 1/CNTN1 is at 113 kDa.
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Figure 2. IF analysis of Contactin 1/CNTN1 using anti-Contactin 1/CNTN1 antibody (A04538-2).
Contactin 1/CNTN1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Contactin 1/CNTN1 Antibody (A04538-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CNTN1 (Source: Uniprot.org, NCBI)
Gene Name
CNTN1
Full Name
Contactin-1
Weight
113.32kDa
Superfamily
immunoglobulin superfamily
Alternative Names
Ras-related protein Rab-11B; GTP-binding protein YPT3; RAB11B; YPT3 CNTN1 F3, GP135, MYPCN contactin 1 contactin-1|glycoprotein gP135|neural cell surface protein F3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CNTN1, check out the CNTN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CNTN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Contactin 1/CNTN1 Antibody Picoband® (A04538-2)
Hello CJ!
A04538-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Increased Expression of Netrin-1 and Its Deleted in Colorectal Cancer Receptor in Human Diseased Lumbar Intervertebral Disc Compared With Autopsy Control
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