Anti-DDX3/DDX3X Antibody Picoband®

DDX3 antibody

Boster Bio Anti-DDX3/DDX3X Antibody Picoband® catalog # A00751-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A00751-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, ICC, WB

Product Name

Anti-DDX3/DDX3X Antibody Picoband®

View all DDX3 Antibodies

SKU/Catalog Number

A00751-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DDX3/DDX3X Antibody Picoband® catalog # A00751-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DDX3/DDX3X Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00751-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human DDX3, identical to the related mouse sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00751-1 is reactive to DDX3X in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

73 kDa

Calculated molecular weight

73243 MW

Background of DDX3

ATP-dependent RNA helicase DDX3X is an enzyme that in humans is encoded by the DDX3X gene. The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00751-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Mouse, Rat

Positive Control

WB: human Hela whole cell, human HepG2 whole cell, human HT1080 whole cell, rat liver tissue, rat C6 whole cell, mouse liver tissue, mouse NIH/3T3 whole cell
ICC/IF: SiHa cell
FCM: A549 cell, ANA-1 cell, C6 cell

Validation Images & Assay Conditions

Gene/Protein Information For DDX3X (Source: Uniprot.org, NCBI)

Gene Name

DDX3X

Full Name

ATP-dependent RNA helicase DDX3X

Weight

73243 MW

Superfamily

DEAD box helicase family

Alternative Names

ATP-dependent RNA helicase DDX3X;3.6.4.13;DEAD box protein 3, X-chromosomal;DEAD box, X isoform;Helicase-like protein 2;HLP2;DDX3X;DBX, DDX3; DDX3X CAP-Rf, DBX, DDX14, DDX3, HLP2, MRX102, MRXSSB DEAD-box helicase 3 X-linked ATP-dependent RNA helicase DDX3X|DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked|DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked|DEAD box protein 3, X-chromosomal|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3|DEAD/H box-3|helicase-like protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DDX3X, check out the DDX3X Infographic

DDX3X infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DDX3X: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-DDX3/DDX3X Antibody Picoband®

Question

We are currently using anti-DDX3/DDX3X antibody A00751-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

N. Patel

Verified customer

Asked: 2014-10-03

Answer

The anti-DDX3/DDX3X antibody (A00751-1) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-10-03

Order DetailsPrice
A00751-1

100μg

$370
A00751-1-10ug

10μg sample (liquid)

$99
A00751-1-Biotin

100 μg Biotin conjugated

$570
A00751-1-Cy3

100 μg Cy3 conjugated

$570
A00751-1-Dylight488

100 μg Dylight488 conjugated

$570
A00751-1-Dylight550

100 μg Dylight550 conjugated

$570
A00751-1-Dylight594

100 μg Dylight594 conjugated

$570
A00751-1-FITC

100 μg FITC conjugated

$570
A00751-1-HRP

100 μg HRP conjugated

$570
A00751-1-APC

100 μg APC conjugated

$670
A00751-1-PE

100 μg PE conjugated

$670
A00751-1-iFluor647

100 μg iFluor647 conjugated

$670
A00751-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A00751-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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