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Product Info Summary
| SKU: | PB9939 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Collagen I/COL1A1 Antibody Picoband®
SKU/Catalog Number
PB9939
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Collagen I/COL1A1 Antibody Picoband® catalog # PB9939. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen I/COL1A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9939)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I, different from the related human sequence by three amino acids, and identical to the related rat sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9939 is reactive to Col1a1 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
138-180 kDa
Calculated molecular weight
138032 MW
Background of COL1A1
Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. And this gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9939 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: mouse NIH/3T3 whole cell
IHC: mouse lung tissue, rat lung tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of COL1A1 using anti-COL1A1 antibody (PB9939).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL1A1 antigen affinity purified polyclonal antibody (Catalog # PB9939) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL1A1 at approximately 138-180 kDa. The expected band size for COL1A1 is at 138 kDa.
Click image to see more details
Figure 2. IHC analysis of COL1A1 using anti COL1A1 antibody (PB9939).
COL1A1 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COL1A1 Antibody (PB9939) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of COL1A1 using anti COL1A1 antibody (PB9939).
COL1A1 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COL1A1 Antibody (PB9939) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Col1a1 (Source: Uniprot.org, NCBI)
Gene Name
Col1a1
Full Name
Collagen alpha-1(I) chain
Weight
138032 MW
Superfamily
fibrillar collagen family
Alternative Names
Collagen alpha-1 (I) chain;Alpha-1 type I collagen;Col1a1;Cola1; COL1A1 CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4 collagen type I alpha 1 chain collagen alpha-1(I) chain|alpha-1 type I collagen|alpha1(I) procollagen|collagen alpha 1 chain type I|collagen alpha-1(I) chain preproprotein|collagen of skin, tendon and bone, alpha-1 chain|collagen, type I, alpha 1|pro-alpha-1 collagen type 1|type I proalpha 1|type I procollagen alpha 1 chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Col1a1, check out the Col1a1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Col1a1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen I/COL1A1 Antibody Picoband® (PB9939)
Hello CJ!
PB9939 has been cited in 59 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Investigating the antifibrotic effect of the antiparasitic drug Praziquantel in in vitro and in vivo preclinical models
Wei X,Bao Y,Zhan X,Zhang L,Hao G,Zhou J,Chen Q.MiR-200a ameliorates peritoneal fibrosis and functional deterioration in a rat model of peritoneal dialysis.Int Urol Nephrol.2019 May;51(5):889-896.doi:10.1007/s11255-019-02122-4.Epub 2019 Mar 19.PMID:30888602;PMCID:PMC6499761.
Species: Rat
PB9939 usage in article: APP:WB, SAMPLE:PERITONEAL TISSUE, DILUTION:NA
Li X,Bu X,Yan F,Wang F,Wei D,Yuan J,Zheng W,Su J,Yuan J.Deletion of discoidin domain receptor 2 attenuates renal interstitial fibrosis in a murine unilateral ureteral obstruction model.Ren Fail.2019 Nov;41(1):481-488.doi:10.1080/0886022X.2019.1621759.PMID:31169440;PMCID:PMC6567249.
Species: Mouse
PB9939 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:NA
Ge Y,Cheng R,Sun S,Zhang S,Li L,Jiang J,Yang C,Xuan X,Chen J.Fangxiao Formula alleviates airway inflammation and remodeling in rats with asthma via suppression of transforming growth factor -β/Smad3 signaling pathway.Biomed Pharmacother.2019 Nov;119:109429.doi:10.1016/j.biopha.2019.109429.Epub 2019 Sep 7.PMID:31505422.
Species: Rat
PB9939 usage in article: APP:IHC, SAMPLE:LUNG TISSUE, DILUTION:NA
Feng Y,Dong H,Sun B,Hu Y,Yang Y,Jia Y,Jia L,Zhong X,Zhao R.METTL3/METTL14 transactivation and m6A-dependent TGF-β1 translation in activated Kupffer cells.Cell Mol Gastroenterol Hepatol.2021 May 13:S2352-345X(21)00096-5.doi:10.1016/j.jcmgh.2021.05.007.Epub ahead of print.PMID:33992834.
Species: Human,Mouse,Rat
PB9939 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:500
Lan Chen,Xiaofang Ji,Manni Wang et al.Involvement of TLR4 Signaling Regulated-COX2/PGE2 Axis in Liver Fibrosis Induced by Schistosoma japonicum Infection, 06 January 2021, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs
Species: Human,Mouse
PB9939 usage in article: APP:IHC, SAMPLE:LIVER TISSUE, DILUTION:1:400
Ma XL,Ding Y,Wu LM,Wang YX,Yao Y,Wang YX,Zhang YG,Niu JQ,He XX,Wang YQ.The glucagon-like peptide-1 (GLP-1) analog exenatide ameliorates intrauterine adhesions in mice.Peptides.2021 Jan 12;137:170481.doi:10.1016/j.peptides.2020.170481.Epub ahead of print.P
Species: Mouse
PB9939 usage in article: APP:WB, SAMPLE:IUA TISSUE, DILUTION:1:1000
Nicotinamide Supplementation Attenuates Renal Interstitial Fibrosis via Boosting the Activity of Sirtuins. Xin Zhen,Shaowu Zhang,Feifei Xie,Miaomiao Zhou,Zheng Hu,Fengxin Zhu,Jing Nie
Species: Mouse
PB9939 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE AND PTCS, DILUTION:NA
Francesca Lombardi,Paola Palumbo,Francesca Rosaria Augello,Ilaria Giusti,Vincenza Dolo,Luca Guerrini,Maria Grazia Cifone,Maurizio Giuliani,Benedetta Cinque,"Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro"
Species: Mouse
PB9939 usage in article: APP:IF, SAMPLE:NIH 3T3 CELL, DILUTION:1:250
Pei-pei Fang,Chen-wei Pan,Wei Lin,Jie Li,Shan-shan Huang,Guang-yao Zhou,Wen-jun Du,Qiang Li, "ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes",Mediators of Inflammation,vol.2020,Art
Species: Human
PB9939 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Collagen I/COL1A1 Antibody Picoband®
Question
I would like using your anti-Collagen I/COL1A1 antibody for response to estradiol studies. Has this antibody been tested with western blotting on mouse lung? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
Thanks for your inquiry. This PB9939 anti-Collagen I/COL1A1 antibody is validated on mouse lung. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-08
Question
I have a question about product PB9939, anti-Collagen I/COL1A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9939 anti-Collagen I/COL1A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-27
Question
Will anti-Collagen I/COL1A1 antibody PB9939 work for IHC with fetal brain cortex?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
According to the expression profile of fetal brain cortex, COL1A1 is highly expressed in fetal brain cortex. So, it is likely that anti-Collagen I/COL1A1 antibody PB9939 will work for IHC with fetal brain cortex.
Boster Scientific Support
Answered: 2019-09-02
Question
See below the WB image, lot number and protocol we used for fetal brain cortex using anti-Collagen I/COL1A1 antibody PB9939. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-07
Question
Do you have a BSA free version of anti-Collagen I/COL1A1 antibody PB9939 available?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Collagen I/COL1A1 antibody PB9939 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-03
Question
We were happy with the WB result of your anti-Collagen I/COL1A1 antibody. However we have been able to see positive staining in fetal brain cortex extracellular using this antibody. Is that expected? Could you tell me where is COL1A1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-03-11
Answer
According to literature, fetal brain cortex does express COL1A1. Generally COL1A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL1A1 expression, here are a few articles citing expression in various tissues:
Bone, Pubmed ID: 3340531
Brain, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Skin, Pubmed ID: 4319110, 5529814
Boster Scientific Support
Answered: 2019-03-11
Question
We are currently using anti-Collagen I/COL1A1 antibody PB9939 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-05
Answer
The anti-Collagen I/COL1A1 antibody (PB9939) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-05
Question
I see that the anti-Collagen I/COL1A1 antibody PB9939 works with IHC, what is the protocol used to produce the result images on the product page?
A. Krishna
Verified customer
Asked: 2019-01-11
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-11
Question
I was wanting to use your anti-Collagen I/COL1A1 antibody for IHC for rat fetal brain cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat fetal brain cortex identification?
Verified Customer
Verified customer
Asked: 2018-09-12
Answer
It shows on the product datasheet, PB9939 anti-Collagen I/COL1A1 antibody has been validated for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fetal brain cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-12
Question
Is this PB9939 anti-Collagen I/COL1A1 antibody reactive to the isotypes of COL1A1?
G. Collins
Verified customer
Asked: 2018-04-03
Answer
The immunogen of PB9939 anti-Collagen I/COL1A1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I (1185-1207aa YDFSFLPQPPQEKSQDGGRYYRA), different from the related human sequence by three amino acids, and identical to the related rat sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-03
Question
We are interested in to test anti-Collagen I/COL1A1 antibody PB9939 on rat fetal brain cortex for research purposes, then I may be interested in using anti-Collagen I/COL1A1 antibody PB9939 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-06
Answer
The products we sell, including anti-Collagen I/COL1A1 antibody PB9939, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-06
Question
Is a blocking peptide available for product anti-Collagen I/COL1A1 antibody (PB9939)?
Verified Customer
Verified customer
Asked: 2017-05-15
Answer
We do provide the blocking peptide for product anti-Collagen I/COL1A1 antibody (PB9939). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-05-15
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain cortex using anti-Collagen I/COL1A1 antibody PB9939. Let me know if you need anything else.
D. Edwards
Verified customer
Asked: 2016-06-29
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-06-29
Question
We have been able to see staining in rat bone. What should we do? Is anti-Collagen I/COL1A1 antibody supposed to stain bone positively?
D. Johnson
Verified customer
Asked: 2014-04-01
Answer
According to literature bone does express COL1A1. According to Uniprot.org, COL1A1 is expressed in spinal cord, spleen, brain, skin, fetal brain cortex, bone, liver, among other tissues. Regarding which tissues have COL1A1 expression, here are a few articles citing expression in various tissues:
Bone, Pubmed ID: 3340531
Brain, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Skin, Pubmed ID: 4319110, 5529814
Boster Scientific Support
Answered: 2014-04-01
Question
We ordered your anti-Collagen I/COL1A1 antibody for WB on skin last year. I am using mouse, and We want to use the antibody for IHC next. My question regards examining skin as well as spinal cord in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
H. Zhao
Verified customer
Asked: 2013-10-28
Answer
I took a look at the website and datasheets of our anti-Collagen I/COL1A1 antibody and it appears that PB9939 has been validated on mouse in both WB and IHC. Thus PB9939 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-10-28
Question
Will PB9939 anti-Collagen I/COL1A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Moore
Verified customer
Asked: 2013-06-07
Answer
You can see on the product datasheet, PB9939 anti-Collagen I/COL1A1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-06-07
