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Product Info Summary
| SKU: | PA1469 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Interleukin-13 IL13 Antibody Picoband®
SKU/Catalog Number
PA1469
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interleukin-13 IL13 Antibody catalog # PA1469. Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-13 IL13 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1469)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of rat IL-13.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1469 is reactive to Il13 in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
13 kDa
Calculated molecular weight
14093 MW
Background of IL-13
IL-13, Interleukin 13, is a protein that in humans is encoded by the IL-13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells that is an important mediator of allergic inflammation and disease. The IL-13 gene is mapped to 5q23-q31. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Ralpha) and at least one of two known IL-13-specific binding chains. IL-13 acts more prominently as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thereby altering physiological function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1469 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat
Validation Images & Assay Conditions
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Anti-IL-13 antibody, PA1469, Western blotting
All lanes: Anti IL-13 (PA1469) at 0.5ug/ml
Lane 1: Recombinant Rat IL-13 Protein 5ng
Lane 2: Recombinant Rat IL-13 Protein 2.5ng
Predicted bind size: 13KD
Observed bind size: 13KD
Protein Target Info & Infographic
Gene/Protein Information For Il13 (Source: Uniprot.org, NCBI)
Gene Name
Il13
Full Name
Interleukin-13
Weight
14093 MW
Superfamily
IL-4/IL-13 family
Alternative Names
Interleukin-13;IL-13;T-cell activation protein P600;Il13;Il-13; IL13 IL-13, P600 interleukin 13 interleukin-13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il13, check out the Il13 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Interleukin-13 IL13 Antibody Picoband® (PA1469)
Hello CJ!
PA1469 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Acute exposure of ozone induced pulmonary injury and the protective role of vitamin E through the Nrf2 pathway in Balb/c mice
Number 2 Feibi Recipe Reduces PM2.5-Induced Lung Injury in Rats
An IL-13 Promoter Polymorphism Associated with Liver Fibrosis in Patients with Schistosoma japonicum
CD68 and interleukin 13, prospective immune markers for esophageal squamous cell carcinoma prognosis prediction
Li J, Li L, Zuo H, Ke C, Yan B, Wen H, Zhang Y, Yang X. Plos One. 2014 Feb 3;9(2):E87887. Doi: 10.1371/Journal.Pone.0087887. Ecollection 2014. T-Helper Type-2 Contact Hypersensitivity Of Balb/C Mice Aggravated By Dibutyl Phthalate Via Long-Term De...
Wang B, Liang S, Wang Y, Zhu Xq, Gong W, Zhang Hq, Li Y, Xia Cm. Plos Negl Trop Dis. 2015 Jan 15;9(1):E0003434. Doi: 10.1371/Journal.Pntd.0003434. Ecollection 2015. Th17 Down-Regulation Is Involved In Reduced Progression Of Schistosomiasis Fibrosi...
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Interleukin-13 IL13 Antibody Picoband®
Question
My team were happy with the WB result of your anti-IL13 antibody. However we have been able to see positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IL13 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
According to literature, blood does express IL13. Generally IL13 expresses in secreted. Regarding which tissues have IL13 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7721105
Peripheral blood lymphocyte, Pubmed ID: 1408833
Boster Scientific Support
Answered: 2019-10-04
Question
We have observed staining in rat left testis. Do you have any suggestions? Is anti-IL13 antibody supposed to stain left testis positively?
Verified Customer
Verified customer
Asked: 2018-07-06
Answer
Based on literature left testis does express IL13. Based on Uniprot.org, IL13 is expressed in left testis, blood, peripheral blood lymphocyte, among other tissues. Regarding which tissues have IL13 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7721105
Peripheral blood lymphocyte, Pubmed ID: 1408833
Boster Scientific Support
Answered: 2018-07-06
Question
We are currently using anti-IL13 antibody PA1469 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-22
Answer
The anti-IL13 antibody (PA1469) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-22
