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Product Info Summary
| SKU: | M08907 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CLIP170 Rabbit Monoclonal Antibody
SKU/Catalog Number
M08907
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-CLIP170 Rabbit Monoclonal Antibody catalog # M08907. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CLIP170 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08907)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
29C06
Isotype
IgG
Immunogen
A synthesized peptide derived from human CLIP170
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M08907 is reactive to CLIP1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
170 kDa
Calculated molecular weight
162 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08907 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: human Hela whole cell, human SiHa whole cell, human A549 whoel cell, human MCF-7 whole cell, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CLIP170 using anti-CLIP170 antibody (M08907).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human A549 whoel cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLIP170 antigen affinity purified monoclonal antibody (M08907) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLIP170 at approximately 170 kDa. The expected band size for CLIP170 is at 162 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CLIP1 (Source: Uniprot.org, NCBI)
Gene Name
CLIP1
Full Name
CAP-Gly domain-containing linker protein 1
Weight
162 kDa
Alternative Names
Cdc42 effector protein 1;Binder of Rho GTPases 5;Serum protein MSE55;CDC42EP1;BORG5, CEP1, MSE55; CLIP1 CLIP, CLIP-17070, CYLN1, RSN, CLIP1 CAP-Gly domain containing linker protein 1 CAP-Gly domain-containing linker protein 1|cytoplasmic linker protein 1|cytoplasmic linker protein 170 alpha-2|cytoplasmic linker protein CLIP-170|restin (Reed-Steinberg cell-expressed intermediate filament-associated protein)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLIP1, check out the CLIP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CLIP170 Rabbit Monoclonal Antibody (M08907)
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