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Product Info Summary
| SKU: | PA1994 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-PDK2 Antibody Picoband®
SKU/Catalog Number
PA1994
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PDK2 Antibody catalog # PA1994. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDK2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1994)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PDK2, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1994 is reactive to PDK2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
85-91 kDa
Calculated molecular weight
46154 MW
Background of PDK2
PDK2 (Pyruvate Dehydrogenase Kinase Isoenzyme 2), is an enzyme that in humans is encoded by the PDK2 gene. This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1994 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Heart Tissue, HELA Cell, SW620 Cell, MCF-7 Cell,
IHC: Human Mammary Cancer Tissue, Rat Brain Tissue
ICC: HELA Cell
Validation Images & Assay Conditions
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Anti-PDK2 antibody, PA1994, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: SW620 Cell Lysate
Lane 4: MCF-7 Cell Lysate
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Anti-PDK2 antibody, PA1994, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Anti-PDK2 antibody, PA1994, IHC(P)
IHC(P): Rat Brain Tissue
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Anti-PDK2 antibody, PA1994, ICC
ICC: HELA Cell
Protein Target Info & Infographic
Gene/Protein Information For PDK2 (Source: Uniprot.org, NCBI)
Gene Name
PDK2
Full Name
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial
Weight
46154 MW
Superfamily
PDK/BCKDK protein kinase family
Alternative Names
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial;2.7.11.2;Pyruvate dehydrogenase kinase isoform 2;PDH kinase 2;PDKII;PDK2;PDHK2; PDK2 PDHK2, PDKII pyruvate dehydrogenase kinase 2 pyruvate dehydrogenase kinase, isozyme 2|PDH kinase 2|[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial|pyruvate dehydrogenase kinase, isoenzyme 2|pyruvate dehydrogenase, lipoamide, kinase isozyme 2, mitochondrial
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDK2, check out the PDK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDK2 Antibody Picoband® (PA1994)
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No publications found for PA1994
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-PDK2 Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-PDK2 antibody PA1994. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-14
Question
Do you have a BSA free version of anti-PDK2 antibody PA1994 available?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PDK2 antibody PA1994 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-31
Question
We are currently using anti-PDK2 antibody PA1994 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
The anti-PDK2 antibody (PA1994) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-19
Question
Does anti-PDK2 antibody PA1994 work for WB with lung?
R. Zhao
Verified customer
Asked: 2014-06-02
Answer
According to the expression profile of lung, PDK2 is highly expressed in lung. So, it is likely that anti-PDK2 antibody PA1994 will work for WB with lung.
Boster Scientific Support
Answered: 2014-06-02
