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Product Info Summary
| SKU: | A00702 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Cleaved-Plasminogen HC A short form (V98) PLG Antibody
SKU/Catalog Number
A00702
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cleaved-Plasminogen HC A short form (V98) PLG Antibody catalog # A00702. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cleaved-Plasminogen HC A short form (V98) PLG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00702)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the N-terminal region of human Plasminogen HC A short form. at AA rangle: 50-130
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00702 is reactive to PLG in Human, Mouse, Rat
Applications
A00702 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
90569 MW
Background of PLG
Anti-Cyclin A detects Cyclin A. Cyclin A is a member of the cyclin family. Cyclin A binds to S phase Cdk2 and is required for the cell to progress through the S phase. Cyclin A/ Cdk2 is inhibited by the complex p21CIP. Anti-Cyclin A Antibody is ideal for investigators involved in Cell Signaling, cell biology and Signal Transduction research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using Cleaved-Plasminogen HC A short form (V98) Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For PLG (Source: Uniprot.org, NCBI)
Gene Name
PLG
Full Name
Plasminogen
Weight
90569 MW
Superfamily
peptidase S1 family
Alternative Names
DKFZp779M0222; EC 3.4.21; EC 3.4.21.7; plasmin; Plasminogen; Plg PLG plasminogen plasminogen|plasmin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLG, check out the PLG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cleaved-Plasminogen HC A short form (V98) PLG Antibody (A00702)
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No publications found for A00702
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Cleaved-Plasminogen HC A short form (V98) PLG Antibody
Question
Does anti-Cleaved-Plasminogen HC A short form (V98) antibody A00702 work for WB with right lobe of liver?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
According to the expression profile of right lobe of liver, PLG is highly expressed in right lobe of liver. So, it is likely that anti-Cleaved-Plasminogen HC A short form (V98) antibody A00702 will work for WB with right lobe of liver.
Boster Scientific Support
Answered: 2020-05-05
Question
Is this A00702 anti-Cleaved-Plasminogen HC A short form (V98) antibody reactive to the isotypes of PLG?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
The immunogen of A00702 anti-Cleaved-Plasminogen HC A short form (V98) antibody is Synthesized peptide derived from the N-terminal region of human Plasminogen HC A short form. at AA rangle: 50-130. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-20
Question
We are currently using anti-Cleaved-Plasminogen HC A short form (V98) antibody A00702 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
The anti-Cleaved-Plasminogen HC A short form (V98) antibody (A00702) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-30
Question
We have observed staining in rat right lobe of liver. Do you have any suggestions? Is anti-Cleaved-Plasminogen HC A short form (V98) antibody supposed to stain right lobe of liver positively?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
According to literature right lobe of liver does express PLG. According to Uniprot.org, PLG is expressed in right lobe of liver, liver, kidney, milk, among other tissues. Regarding which tissues have PLG expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 14574404, 24275569
Milk, Pubmed ID: 18780401
Boster Scientific Support
Answered: 2019-10-16
Question
Would A00702 anti-Cleaved-Plasminogen HC A short form (V98) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
You can see on the product datasheet, A00702 anti-Cleaved-Plasminogen HC A short form (V98) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-10
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lobe of liver using anti-Cleaved-Plasminogen HC A short form (V98) antibody A00702. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-05
Question
My question regarding product A00702, anti-Cleaved-Plasminogen HC A short form (V98) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Krishna
Verified customer
Asked: 2019-07-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00702 anti-Cleaved-Plasminogen HC A short form (V98) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-19
Question
Is there a BSA free version of anti-Cleaved-Plasminogen HC A short form (V98) antibody A00702 available?
S. Jones
Verified customer
Asked: 2017-05-19
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Cleaved-Plasminogen HC A short form (V98) antibody A00702 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-05-19
Question
See attached the WB image, lot number and protocol we used for right lobe of liver using anti-Cleaved-Plasminogen HC A short form (V98) antibody A00702. Please let me know if you require anything else.
M. Singh
Verified customer
Asked: 2017-01-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-01-03
Question
I was wanting to use your anti-Cleaved-Plasminogen HC A short form (V98) antibody for WB for mouse right lobe of liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse right lobe of liver identification?
J. Banerjee
Verified customer
Asked: 2015-11-10
Answer
It shows on the product datasheet, A00702 anti-Cleaved-Plasminogen HC A short form (V98) antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse right lobe of liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-11-10
Question
Is a blocking peptide available for product anti-Cleaved-Plasminogen HC A short form (V98) antibody (A00702)?
J. Evans
Verified customer
Asked: 2014-03-14
Answer
We do provide the blocking peptide for product anti-Cleaved-Plasminogen HC A short form (V98) antibody (A00702). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-03-14
Question
Our team were content with the WB result of your anti-Cleaved-Plasminogen HC A short form (V98) antibody. However we have observed positive staining in milk secreted using this antibody. Is that expected? Could you tell me where is PLG supposed to be expressed?
K. Lewis
Verified customer
Asked: 2013-11-28
Answer
According to literature, milk does express PLG. Generally PLG expresses in secreted. Regarding which tissues have PLG expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 14574404, 24275569
Milk, Pubmed ID: 18780401
Boster Scientific Support
Answered: 2013-11-28
Question
I am interested in to test anti-Cleaved-Plasminogen HC A short form (V98) antibody A00702 on mouse right lobe of liver for research purposes, then I may be interested in using anti-Cleaved-Plasminogen HC A short form (V98) antibody A00702 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Gonzalez
Verified customer
Asked: 2013-07-24
Answer
The products we sell, including anti-Cleaved-Plasminogen HC A short form (V98) antibody A00702, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-07-24
Question
I see that the anti-Cleaved-Plasminogen HC A short form (V98) antibody A00702 works with WB, what is the protocol used to produce the result images on the product page?
Z. Wu
Verified customer
Asked: 2013-05-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-05-06
