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Product Info Summary
| SKU: | A09156 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-CLCN4/Clc 4 Antibody
SKU/Catalog Number
A09156
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CLCN4/Clc 4 Antibody (Catalog # A09156). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CLCN4/Clc 4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09156)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CLCN4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09156 is reactive to CLCN4 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
84917 MW
Background of CLCN4
Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons.
van Slegtenhorst M.A., Hum. Mol. Genet. 3:547-552(1994).
Kawasaki M., Am. J. Physiol. 277:C948-C954(1999).
Rae J.L.; Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09156 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from MCF-7 cells, using CLCN4 antibody A09156.
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Immunofluorescence analysis of HUVEC cells, using CLCN4 antibody A09156.
Protein Target Info & Infographic
Gene/Protein Information For CLCN4 (Source: Uniprot.org, NCBI)
Gene Name
CLCN4
Full Name
H(+)/Cl(-) exchange transporter 4
Weight
84917 MW
Superfamily
chloride channel (TC 2.A.49) family
Alternative Names
H (+)/Cl (-) exchange transporter 4;Chloride channel protein 4;ClC-4;Chloride transporter ClC-4;CLCN4; CLCN4 CLC4, ClC-4, ClC-4A, MRX15, MRX49, MRXSRC chloride voltage-gated channel 4 H(+)/Cl(-) exchange transporter 4|chloride channel 4|chloride channel protein 4|chloride channel, voltage-sensitive 4|chloride transporter ClC-4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLCN4, check out the CLCN4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLCN4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CLCN4/Clc 4 Antibody (A09156)
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No publications found for A09156
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-CLCN4/Clc 4 Antibody
Question
We want to test anti-CLCN4/Clc 4 antibody A09156 on rat brain for research purposes, then I may be interested in using anti-CLCN4/Clc 4 antibody A09156 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
The products we sell, including anti-CLCN4/Clc 4 antibody A09156, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-19
Question
We are currently using anti-CLCN4/Clc 4 antibody A09156 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
The anti-CLCN4/Clc 4 antibody (A09156) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-15
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-CLCN4/Clc 4 antibody A09156. Let me know if you need anything else.
G. Huang
Verified customer
Asked: 2019-02-07
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-07
Question
I was wanting to use your anti-CLCN4/Clc 4 antibody for WB for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?
G. Walker
Verified customer
Asked: 2018-11-22
Answer
It shows on the product datasheet, A09156 anti-CLCN4/Clc 4 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-22
Question
See attached the WB image, lot number and protocol we used for brain using anti-CLCN4/Clc 4 antibody A09156. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-08-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-07
Question
Is a blocking peptide available for product anti-CLCN4/Clc 4 antibody (A09156)?
C. Lewis
Verified customer
Asked: 2017-04-11
Answer
We do provide the blocking peptide for product anti-CLCN4/Clc 4 antibody (A09156). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-04-11
