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Product Info Summary
| SKU: | A01350 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Galectin 10/CLC Antibody Picoband®
View all Galectin-10 Antibodies
SKU/Catalog Number
A01350
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Galectin 10/CLC Antibody Picoband® catalog # A01350. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Galectin 10/CLC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01350)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Galectin 10 recombinant protein (Position: S2-R142).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01350 is reactive to CLC in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
16 kDa
Calculated molecular weight
16.453kDa
Background of Galectin-10
Eosinophil lysophospholipase is an enzyme that in humans is encoded by the CLC gene. Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene is a lysophospholipase expressed in eosinophils and basophils. It hydrolyzes lysophosphatidylcholine to glycerophosphocholine and a free fatty acid. This protein may possess carbohydrate or IgE-binding activities. It is both structurally and functionally related to the galectin family of beta-galactoside binding proteins. It may be associated with inflammation and some myeloid leukemias.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01350 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human HL-60 whole cell, human PANC-1 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Galectin 10 using anti-Galectin 10 antibody (A01350).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human PANC-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 10 antigen affinity purified polyclonal antibody (Catalog # A01350) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 10 at approximately 16KD. The expected band size for Galectin 10 is at 16KD.
Protein Target Info & Infographic
Gene/Protein Information For CLC (Source: Uniprot.org, NCBI)
Gene Name
CLC
Full Name
Galectin-10
Weight
16.453kDa
Alternative Names
Galectin-10; Gal-10; Charcot-Leyden crystal protein; CLC; Eosinophil lysophospholipase; Lysolecithin acylhydrolase; CLC; LGALS10; LGALS10A CLC GAL10, Gal-10, LGALS10, LGALS10A, LPPL_HUMAN Charcot-Leyden crystal galectin galectin-10|Charcot-Leyden crystal protein|eosinophil lysophospholipase|lectin, galactoside-binding, soluble, 10|lysolecithin acylhydrolase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLC, check out the CLC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Galectin 10/CLC Antibody Picoband® (A01350)
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No publications found for A01350
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Customer Q&As
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7 Customer Q&As for Anti-Galectin 10/CLC Antibody Picoband®
Question
We are currently using anti-Galectin 10/CLC antibody A01350 for human tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
T. Brown
Verified customer
Asked: 2020-05-04
Answer
The anti-Galectin 10/CLC antibody (A01350) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-04
Question
I am interested in to test anti-Galectin 10/CLC antibody A01350 on human placenta for research purposes, then I may be interested in using anti-Galectin 10/CLC antibody A01350 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
The products we sell, including anti-Galectin 10/CLC antibody A01350, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-07
Question
Here is the WB image, lot number and protocol we used for placenta using anti-Galectin 10/CLC antibody A01350. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-21
Question
Does anti-Galectin 10/CLC antibody A01350 work on monkey ELISA with liver?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
Our lab technicians have not tested anti-Galectin 10/CLC antibody A01350 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Galectin 10/CLC antibody A01350 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-08
Question
Is a blocking peptide available for product anti-Galectin 10/CLC antibody (A01350)?
D. Jones
Verified customer
Asked: 2019-10-02
Answer
We do provide the blocking peptide for product anti-Galectin 10/CLC antibody (A01350). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-02
Question
I have a question about product A01350, anti-Galectin 10/CLC antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Wu
Verified customer
Asked: 2016-01-29
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01350 anti-Galectin 10/CLC antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-01-29
Question
Is there a BSA free version of anti-Galectin 10/CLC antibody A01350 available?
C. Roberts
Verified customer
Asked: 2015-05-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Galectin 10/CLC antibody A01350 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-05-29
