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Key=meta_title, value=SLC9A2/Sodium/hydrogen exchanger 2 Antibody
Key=meta_description, value=Boster Bio Anti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody catalog # PA2219. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody.
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Key=image_label, value=Figure 2. IHC analysis of SLC9A2 using anti-SLC9A2 antibody (PA2219).
SLC9A2 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution)
Key=small_image_label, value=Figure 2. IHC analysis of SLC9A2 using anti-SLC9A2 antibody (PA2219).
SLC9A2 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution)
Key=thumbnail_label, value=Figure 2. IHC analysis of SLC9A2 using anti-SLC9A2 antibody (PA2219).
SLC9A2 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution)
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Key=size, value=100 μg/vial
Key=protein_name, value=Sodium/hydrogen exchanger 2
Key=gene_full_name, value=Sodium/hydrogen exchanger 2
Key=uniprot_id, value=Q9UBY0
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Key=sensitivity
Key=applications, value=IHC, WB
Key=reactivity, value=Human, Mouse, Rat
Key=form, value=Lyophilized
Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Key=predicted_reactivity
Key=source_company, value=Boster PA2219
Key=isotype, value=Rabbit IgG
Key=molecular_weight, value=91520 MW
Key=sample_size_available, value=30ug for $99, contact us for details
Key=related_product_skus, value=B0001-5,AR1156,AR0146
Key=product_category, value=Primary Antibodies
Key=research_category, value=Cardiovascular, Cell Biology, Protease Inhibitors, Proteolysis/Ubiquitin
Key=clonality, value=Polyclonal
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Key=cj_related_products, value=Protein Biotinylation:A05440|A06783|RP1059|PB9684|M03090
Key=description, value=Boster Bio Anti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody catalog # PA2219. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody catalog # PA2219. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody
Key=contents, value=Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Key=background, value=Sodium–hydrogen exchanger 2, also called SLC9A2 or NHE2 is a protein that in humans is encoded by the SLC9A2 gene. This gene is mapped to 2q12.1. The Na+/H+ exchangers (NHE) are membrane proteins involved in cell volume. The exchanger (which they called NHE2) is found in several tissues, including intestine and kidney, and is highly expressed in villus and distal convoluted tubules. This gene is involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. It seems to play an important role in colonic sodium absorption.
Key=gene_name, value=SLC9A2
Key=synonyms, value=Sodium/hydrogen exchanger 2;Na (+)/H (+) exchanger 2;NHE-2;Solute carrier family 9 member 2;SLC9A2;NHE2;
Key=application_details, value=Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat

Key=subcellular_localization, value= Membrane; Multi-pass membrane protein.
Key=sequence_similarities, value=Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
Key=tissue_specificity, value= Expressed in skeletal muscle, colon and kidney. Lower levels in the testis, prostate, ovary, and small intestine.
Key=protein_function, value= Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Seems to play an important role in colonic sodium absorption.
Key=application_notes, value=Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 22928|sc 30167
Key=immunogen, value=A synthetic peptide corresponding to a sequence at the C-terminus of human SLC9A2, different from the related rat and mouse sequences by two amino acids.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=91 kDa
Key=custom_attribute_2, value=WB: rat kidney tissue, mouse kidney tissue, mouse kidney tissue
IHC: human lung cancer tissue, rat kidney tissue
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Anti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody Picoband®

SLC9A2 antibody

Boster Bio Anti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody catalog # PA2219. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PA2219
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody Picoband®

View all SLC9A2 Antibodies

SKU/Catalog Number

PA2219

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody catalog # PA2219. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2219)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SLC9A2, different from the related rat and mouse sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2219 is reactive to SLC9A2 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

91 kDa

Calculated molecular weight

91520 MW

Background of SLC9A2

Sodium–hydrogen exchanger 2, also called SLC9A2 or NHE2 is a protein that in humans is encoded by the SLC9A2 gene. This gene is mapped to 2q12.1. The Na+/H+ exchangers (NHE) are membrane proteins involved in cell volume. The exchanger (which they called NHE2) is found in several tissues, including intestine and kidney, and is highly expressed in villus and distal convoluted tubules. This gene is involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. It seems to play an important role in colonic sodium absorption.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA2219 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat

Positive Control

WB: rat kidney tissue, mouse kidney tissue, mouse kidney tissue
IHC: human lung cancer tissue, rat kidney tissue

Validation Images & Assay Conditions

Gene/Protein Information For SLC9A2 (Source: Uniprot.org, NCBI)

Gene Name

SLC9A2

Full Name

Sodium/hydrogen exchanger 2

Weight

91520 MW

Superfamily

monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family

Alternative Names

Sodium/hydrogen exchanger 2;Na (+)/H (+) exchanger 2;NHE-2;Solute carrier family 9 member 2;SLC9A2;NHE2; SLC9A2 NHE2 solute carrier family 9 member A2 sodium/hydrogen exchanger 2|Na(+)/H(+) exchanger 2|solute carrier family 9 (sodium/hydrogen exchanger), member 2|solute carrier family 9, subfamily A (NHE2, cation proton antiporter 2), member 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SLC9A2, check out the SLC9A2 Infographic

SLC9A2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SLC9A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody Picoband®

Question

We are currently using anti-SLC9A2 antibody PA2219 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

The anti-SLC9A2 antibody (PA2219) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-26

Order DetailsPrice
PA2219

100μg

$370
PA2219-10ug

10μg sample (liquid)

$99
PA2219-Biotin

100 μg Biotin conjugated

$570
PA2219-Cy3

100 μg Cy3 conjugated

$570
PA2219-Dylight488

100 μg Dylight488 conjugated

$570
PA2219-Dylight550

100 μg Dylight550 conjugated

$570
PA2219-Dylight594

100 μg Dylight594 conjugated

$570
PA2219-FITC

100 μg FITC conjugated

$570
PA2219-HRP

100 μg HRP conjugated

$570
PA2219-APC

100 μg APC conjugated

$670
PA2219-PE

100 μg PE conjugated

$670
PA2219-iFluor647

100 μg iFluor647 conjugated

$670
PA2219-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PA2219
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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