Click image to see more details
Product Info Summary
| SKU: | PA2219 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody Picoband®
SKU/Catalog Number
PA2219
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody catalog # PA2219. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2219)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SLC9A2, different from the related rat and mouse sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2219 is reactive to SLC9A2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
91 kDa
Calculated molecular weight
91520 MW
Background of SLC9A2
Sodium–hydrogen exchanger 2, also called SLC9A2 or NHE2 is a protein that in humans is encoded by the SLC9A2 gene. This gene is mapped to 2q12.1. The Na+/H+ exchangers (NHE) are membrane proteins involved in cell volume. The exchanger (which they called NHE2) is found in several tissues, including intestine and kidney, and is highly expressed in villus and distal convoluted tubules. This gene is involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. It seems to play an important role in colonic sodium absorption.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2219 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Positive Control
WB: rat kidney tissue, mouse kidney tissue, mouse kidney tissue
IHC: human lung cancer tissue, rat kidney tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SLC9A2 using anti-SLC9A2 antibody (PA2219).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC9A2 antigen affinity purified polyclonal antibody (Catalog # PA2219) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC9A2 at approximately 91KD. The expected band size for SLC9A2 is at 91KD.
Click image to see more details
Figure 2. IHC analysis of SLC9A2 using anti-SLC9A2 antibody (PA2219).
SLC9A2 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC9A2 Antibody (PA2219) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of SLC9A2 using anti-SLC9A2 antibody (PA2219).
SLC9A2 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC9A2 Antibody (PA2219) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. Western blot analysis of SLC9A2 using anti-SLC9A2 antibody (PA2219).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse kidney tissue lysates,
Lane 2: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC9A2 antigen affinity purified polyclonal antibody (Catalog # PA2219) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC9A2 at approximately 91KD. The expected band size for SLC9A2 is at 91KD.
Protein Target Info & Infographic
Gene/Protein Information For SLC9A2 (Source: Uniprot.org, NCBI)
Gene Name
SLC9A2
Full Name
Sodium/hydrogen exchanger 2
Weight
91520 MW
Superfamily
monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family
Alternative Names
Sodium/hydrogen exchanger 2;Na (+)/H (+) exchanger 2;NHE-2;Solute carrier family 9 member 2;SLC9A2;NHE2; SLC9A2 NHE2 solute carrier family 9 member A2 sodium/hydrogen exchanger 2|Na(+)/H(+) exchanger 2|solute carrier family 9 (sodium/hydrogen exchanger), member 2|solute carrier family 9, subfamily A (NHE2, cation proton antiporter 2), member 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC9A2, check out the SLC9A2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC9A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody Picoband® (PA2219)
Hello CJ!
No publications found for PA2219
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody Picoband®
Question
We are currently using anti-SLC9A2 antibody PA2219 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
The anti-SLC9A2 antibody (PA2219) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-26
