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Product Info Summary
| SKU: | M03260 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Claudin 5 CLDN5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03260
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Claudin 5 CLDN5 Rabbit Monoclonal Antibody catalog # M03260. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Claudin 5 CLDN5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03260)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAHA-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Claudin 5
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03260 is reactive to CLDN5 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
130 kDa
Calculated molecular weight
23147 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03260 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Claudin 5 expression in human fetal brain lysate.
Protein Target Info & Infographic
Gene/Protein Information For CLDN5 (Source: Uniprot.org, NCBI)
Gene Name
CLDN5
Full Name
Claudin-5
Weight
23147 MW
Superfamily
claudin family
Alternative Names
Claudin-5;Transmembrane protein deleted in VCFS;TMDVCF;CLDN5;AWAL, TMVCF; CLDN5 AWAL, BEC1, CPETRL1, TMDVCF, TMVCF claudin 5 claudin-5|transmembrane protein deleted in VCFS|transmembrane protein deleted in velocardiofacial syndrome
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLDN5, check out the CLDN5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLDN5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Claudin 5 CLDN5 Rabbit Monoclonal Antibody (M03260)
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Customer Q&As
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14 Customer Q&As for Anti-Claudin 5 CLDN5 Rabbit Monoclonal Antibody
Question
I have attached the WB image, lot number and protocol we used for brain lung using anti-Claudin 5 Rabbit Monoclonal antibody M03260. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-09
Question
We are currently using anti-Claudin 5 Rabbit Monoclonal antibody M03260 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
The anti-Claudin 5 Rabbit Monoclonal antibody (M03260) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-28
Question
Do you have a BSA free version of anti-Claudin 5 Rabbit Monoclonal antibody M03260 available?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Claudin 5 Rabbit Monoclonal antibody M03260 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-13
Question
Our lab want to know about to test anti-Claudin 5 Rabbit Monoclonal antibody M03260 on human brain lung for research purposes, then I may be interested in using anti-Claudin 5 Rabbit Monoclonal antibody M03260 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Miller
Verified customer
Asked: 2019-09-30
Answer
The products we sell, including anti-Claudin 5 Rabbit Monoclonal antibody M03260, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-30
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain lung using anti-Claudin 5 Rabbit Monoclonal antibody M03260. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-19
Question
Can you help my question with product M03260, anti-Claudin 5 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03260 anti-Claudin 5 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-26
Question
I was wanting to use your anti-Claudin 5 Rabbit Monoclonal antibody for WB for human brain lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain lung identification?
Verified Customer
Verified customer
Asked: 2018-09-03
Answer
You can see on the product datasheet, M03260 anti-Claudin 5 Rabbit Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-03
Question
Is a blocking peptide available for product anti-Claudin 5 Rabbit Monoclonal antibody (M03260)?
Verified Customer
Verified customer
Asked: 2018-04-06
Answer
We do provide the blocking peptide for product anti-Claudin 5 Rabbit Monoclonal antibody (M03260). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-04-06
Question
We have been able to see staining in human right lung. Are there any suggestions? Is anti-Claudin 5 Rabbit Monoclonal antibody supposed to stain right lung positively?
Verified Customer
Verified customer
Asked: 2017-11-27
Answer
According to literature right lung does express CLDN5. According to Uniprot.org, CLDN5 is expressed in right lung, prostate, brain lung, among other tissues. Regarding which tissues have CLDN5 expression, here are a few articles citing expression in various tissues:
Brain, and Lung, Pubmed ID: 15489334
Prostate, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-11-27
Question
I see that the anti-Claudin 5 Rabbit Monoclonal antibody M03260 works with WB, what is the protocol used to produce the result images on the product page?
D. Miller
Verified customer
Asked: 2016-05-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-05-09
Question
Does anti-Claudin 5 Rabbit Monoclonal antibody M03260 work for WB with brain lung?
A. Singh
Verified customer
Asked: 2016-05-05
Answer
According to the expression profile of brain lung, CLDN5 is highly expressed in brain lung. So, it is likely that anti-Claudin 5 Rabbit Monoclonal antibody M03260 will work for WB with brain lung.
Boster Scientific Support
Answered: 2016-05-05
Question
My team were satisfied with the WB result of your anti-Claudin 5 Rabbit Monoclonal antibody. However we have been able to see positive staining in prostate cell junction using this antibody. Is that expected? Could you tell me where is CLDN5 supposed to be expressed?
B. Jones
Verified customer
Asked: 2015-05-11
Answer
From literature, prostate does express CLDN5. Generally CLDN5 expresses in cell junction, tight junction. cell. Regarding which tissues have CLDN5 expression, here are a few articles citing expression in various tissues:
Brain, and Lung, Pubmed ID: 15489334
Prostate, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-05-11
Question
Is this M03260 anti-Claudin 5 Rabbit Monoclonal antibody reactive to the isotypes of CLDN5?
A. Jha
Verified customer
Asked: 2015-01-30
Answer
The immunogen of M03260 anti-Claudin 5 Rabbit Monoclonal antibody is A synthesized peptide derived from human Claudin 5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-01-30
Question
Will M03260 anti-Claudin 5 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Wu
Verified customer
Asked: 2013-08-30
Answer
It shows on the product datasheet, M03260 anti-Claudin 5 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-08-30
