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Product Info Summary
| SKU: | PB9506 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SOCS4 Antibody Picoband®
SKU/Catalog Number
PB9506
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SOCS4 Antibody Picoband® catalog # PB9506. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SOCS4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9506)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SOCS4 recombinant protein (Position: M291-C440). Human SOCS4 shares 98% amino acid (aa) sequence identity with mouse SOCS4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9506 is reactive to SOCS4 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
51 kDa
Calculated molecular weight
50623 MW
Background of SOCS-4
SOCS4, also known as Suppressor of cytokine signaling 4, is mapped to 14q22.1. The protein encoded by this gene contains a SH2 domain and a SOCS BOX domain. The protein thus belongs to the suppressor of cytokine signaling (SOCS), also known as STAT-induced STAT inhibitor (SSI), protein family. SOCS family members are known to be cytokine-inducible negative regulators of cytokine signaling. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9506 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: MCF-7 Whole Cell, SW620 Whole Cell, HELA Whole Cell, SKOV Whole Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SOCS4 using anti-SOCS4 antibody (PB9506).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: MCF-7 Whole Cell Lysate at 40ug,
Lane 2: SW620 Whole Cell Lysate at 40ug,
Lane 3: HELA Whole Cell Lysate at 40ug,
Lane 4: SKOV Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS4 antigen affinity purified polyclonal antibody (Catalog # PB9506) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOCS4 at approximately 51 kDa. The expected band size for SOCS4 is at 51 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SOCS4 (Source: Uniprot.org, NCBI)
Gene Name
SOCS4
Full Name
Suppressor of cytokine signaling 4
Weight
50623 MW
Alternative Names
Suppressor of cytokine signaling 4;SOCS-4;Suppressor of cytokine signaling 7;SOCS-7;SOCS4;SOCS7; Socs4|3110032M18Rik, A730004F22Rik, AI427843, AU015727, AU015859, So, Socs7|suppressor of cytokine signaling 4|suppressor of cytokine signaling 4|SOCS-4|SOCS-7|suppressor of cytokine signaling 7|suppressor of cytokine signalling 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOCS4, check out the SOCS4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOCS4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SOCS4 Antibody Picoband® (PB9506)
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Customer Q&As
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1 Customer Q&As for Anti-SOCS4 Antibody Picoband®
Question
We are currently using anti-SOCS4 antibody PB9506 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
A. Miller
Verified customer
Asked: 2018-01-04
Answer
The anti-SOCS4 antibody (PB9506) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-04
