Claudin-5 Antibodies

Claudin-5 (CLDN5)

Plays a major role in tight junction-specific obliteration of the intercellular space. .

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Gene Information

Human
Gene Name:	CLDN5
Uniprot:	O00501
Entrez:	7122

Family

Belongs to:
claudin family

Alternative Names

AWAL; AWALTransmembrane protein deleted in VCFS; BEC1; claudin 5; Claudin5; Claudin-5; CLDN5; CPETRL1; TMDVCF; TMVCF1; TMVCFTMDVCF; transmembrane protein deleted in velocardiofacial syndrome

Molecular Weight

Mass (kDA):
23.147 kDA

Genomic Context

Human
Location:	22q11.21
Sequence:	22; NC_000022.11 (19523024..19525337, complement)

Subcellular Localizations

Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.

Diseases

• Tissue Adhesions
• Edema
• Neoplasms
• Malignant Neoplasms
• Inflammation
• Cerebrovascular Accident
• Ischemia
• Brain Edema
• Carcinoma
• Brain Ischemia
• Nervousness
• Arterial Occlusion

• Extravasation
• Cerebral Artery Occlusion
• Occlusion, Middle Cerebral Artery
• Infarction
• Adenocarcinoma
• Malignant Paraganglionic Neoplasm
• Infarction, Middle Cerebral Artery

Pathways

• Localization
• Transport
• Pathogenesis
• Cell Adhesion
• Neuroprotection
• Inflammatory Response
• Methylation
• Tight Junction Assembly
• Dna Methylation
• Angiogenesis
• Cell Activation
• Cell-cell Adhesion

• Regeneration
• Brain Development
• Reverse Transcription
• Cell Death
• Cell Migration
• Cell Motility
• Cell-matrix Adhesion

PTMs

• Phosphorylation
• Methylation
• Demethylation
• Nitration

• Cleavage
• Acetylation
• Oxidation
• Ubiquitination

Research Areas

• Cellular Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CLDN5

Best Uses Of The Anti-Claudin-5/CLDN5 Marker In Immunohistochemistry

This article will discuss the use of Anti-Claudin-5/CLDN5 Marker and the Immunohistochemistry methods. The methods described in this article can be used by scientists for different purposes including species, applications, and special samples. All scientists can use this marker for research. It is applicable to scientists across the world. To learn more, read on! Here are some of the best uses of the CLDN5 Marker.

Anti-Claudin-5/CLDN5 Marker

The Anti-Claudin-5/CLDN-5 Marker in Boster Bio reacts with Human. The antibody was validated in IHC, ICC, and immunofluorescence. It was designed to recognize Human and Mouse Claudin-5. This antibody can be used in both conventional and non-invasive immunohistochemistry. Boster Bio provides the highest quality antibodies.

Cldn5 is an endothelial-specific member of the claudin family. It is regulated by the Wnt/b-catenin pathway. Cldn5 plays a vital role in the formation of TJs in brain ECs. It has also been implicated in paracellular permeability. Cldn3 and -12 may also contribute to the formation of the BBB.

A defect in Angpt2 results in focal hotspot leakage sites. This defect occurs in endothelial cells with altered gene expression. In Angpt2-deficient mice, a claudin-containing tight junctions is weakened. Angpt2 is a central mediator of the BBB, so when it is decreased or missing, a leaky BBB occurs.

Immunohistochemistry

To obtain the CLDN5 marker, you will need an anti-Claudin 5 antibody. This specific antibody reacts with Human, Mouse and Rat. It is recommended to store this product at -20°C. It is also available frozen. It is also available for research purposes. Here's a list of its best uses. It's useful for determining the levels of CLDN5 in different tissues and cells.

Histochemistry

The presence of CLDN5 in the FS suggests that CLDN5 expression is associated with foot processes that are injured. The CLDN5 marker in FS is generally expressed in the cell-cell junctions of effaced foot processes of NTS-injected mice. The expression of CLDN5 increases with FSD. It is thus possible to define CLDN5 as an early effacement marker. Its localization might be a precursor to the effacement process and deposition of extracellular matrix.

Moreover, CLDN5 is co-expressed with nephrin. Human sections show the formation of tight junctions between podocyte cells. When nephrin is present, CLDN5 expression increases, and the glomerular slit diaphragm remains luminal. However, CLDN5 expression was up-regulated in FS compared to endothelial cells.

Histochemistry of the CLDN5 marker involves measuring the levels of claudin-5 protein in cells. Cells expressing the wild-type variant expressed 50% less claudin-5 than those expressing the rs10314 variant. However, cells with the empty-vector-expression control expressed claudin-5 with a 75% lower expression than those with the wild-type variant. The claudin-5 protein is expressed mostly in the perinnuclear bodies and the BBB. Interestingly, lysosomes were also positively stained by the variant claudin-5 protein.

Claudin-5 is a promising new marker in hemangioendotheliomas and angiosarcomas. Although it is not a universal marker, it is highly expressed in many types of cancers, including the common sarcomas. For cancer diagnosis, claudin-5 expression in vascular tumors should be assessed in the context of a broader antibody panel including keratins and the epithelial membrane antigen.

Immunocytochemistry

CLDN5 is a protein associated with the injury of foot processes. It has recently been discovered that CLDN5 is present in intact filtration slits, where it may be located in non-TJ cell-cell contact. These data suggest that CLDN5 may initiate foot process effacement in these areas. Further, Ichimura et al.13 demonstrated that CLDN5 was localized in a distinct vertical distance between adjacent slit diaphragms, a finding that suggests that CLDN5 is associated with slit-diaphragm junctions.

In the present study, CLDN5 was co-expressed with nephrin. The formation of tight junctions was observed in the podocyte-cell contact. This result suggests that CLDN5 is up-regulated during the podocyte-cell contact. The distal lung contains lymphatic capillaries, bronchi, and large vascular vessels. Lymphatic ECs are known to express PDPN, a protein involved in the maturation of lymphatic vessels. The CLDN5-specific marker genes include the transcription factors and receptors of the semaphorin-related protein.