Product Info Summary
SKU: | A00564-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Fibronectin/FN1 Antibody Picoband®
View all Fibronectin Antibodies
SKU/Catalog Number
A00564-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fibronectin/FN1 Antibody Picoband® catalog # A00564-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fibronectin/FN1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00564-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
Purified human Fibronectin derived.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00564-1 is reactive to FN1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
220-400 kDa
Calculated molecular weight
272.32kDa
Background of Fibronectin
Fibronectin is a high-molecular weight glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. It is mapped to 2q35. This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00564-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human placenta tissue, human hepatocellular carcinoma tumor tissue (HCCT), human hepatocellular carcinoma paracancerous tissue (HCCP)
IHC: human adrenocortical adenoma tissue, human colorectal adenocarcinoma tissue
FCM: CACO-2 cell, HeLa cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FN1 using anti-FN1 antibody (A00564-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FN1 antigen affinity purified polyclonal antibody (Catalog # A00564-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FN1 at approximately 220-400 kDa. The expected band size for FN1 is at 272 kDa.
Click image to see more details
4. Flow Cytometry analysis of CACO-2 cells using anti-FN1 antibody (A00564-1).
Overlay histogram showing CACO-2 cells stained with A00564-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FN1 Antibody (A00564-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 2. IHC analysis of FN1 using anti-FN1 antibody (A00564-1).
FN1 was detected in a paraffin-embedded section of human adrenocortical adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FN1 Antibody (A00564-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
5. Flow Cytometry analysis of HeLa cells using anti-FN1 antibody (A00564-1).
Overlay histogram showing HeLa cells stained with A00564-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FN1 Antibody (A00564-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 3. IHC analysis of FN1 using anti-FN1 antibody (A00564-1).
FN1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FN1 Antibody (A00564-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For FN1 (Source: Uniprot.org, NCBI)
Gene Name
FN1
Full Name
Fibronectin
Weight
272.32kDa
Alternative Names
Fibronectin; FN; Cold-insoluble globulin; CIG; Anastellin; Ugl-Y1; Ugl-Y2; Ugl-Y3; FN1; FN FN1 CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF fibronectin 1 fibronectin|cold-insoluble globulin|epididymis secretory sperm binding protein|migration-stimulating factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FN1, check out the FN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fibronectin/FN1 Antibody Picoband® (A00564-1)
Hello CJ!
A00564-1 has been cited in 18 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Fibronectin-/fibrinogen-binding protein (FBPS) is not a critical virulence factor for the Streptococcus suis serotype 2 strain ZY05719
Yang, Y., Deng, R., Chen, Z., Yao, L., Yang, X., & Xiang, D. (2021). Piperazine ferulate attenuates high glucose‑induced mesangial cell injury via the regulation of p66Shc. Molecular Medicine Reports, 23, 374. https://doi.org/10.3892/mmr.2021.12013
Species: Mouse
A00564-1 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:500
Lian Y,Zhu M,Chen J,Yang B,Lv Q,Wang L,Guo S,Tan X,Li C,Bu W,Ding W,Jia X,Feng L. Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy. Int J Biol Macromol. 2021 Feb 10:S0141-8130(2
Species: Human,Rat
A00564-1 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:200
Zhang X,Ke PX,Yuan X,Zhang GP,Chen WL,Zhang GS. Forskolin Protected against Streptozotocin-Induced Diabetic Cardiomyopathy via Inhibition of Oxidative Stress and Cardiac Fibrosis in Mice. Biomed Res Int.2021 Jan 29;2021:8881843.doi:10.1155/2021/8881843.PM
Species: Mouse
A00564-1 usage in article: APP:WB, SAMPLE:VENTRICULAR TISSUE, DILUTION:NA
Jue-Ying Chen,Ji-Wei Qiu,Jun-Lin Mo,et al.Effects of Yishenbupi (tonifying-kidney and invigorating-spleen) prescription on the expression of renal fibrosis-associated proteins in unilateral ureteral occlusion rats.TMR Integrative Medicine 2020,4:e20012.
Species: Rat
A00564-1 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION1:1000
Xu L,Sun N,Li G,Liu L.LncRNA H19 promotes keloid formation through targeting the miR-769-5p/EIF3A pathway.Mol Cell Biochem.2021 Jan 3.doi:10.1007/s11010-020-04024-x.Epub ahead of print.PMID:33389493.
Species: Human
A00564-1 usage in article: APP:WB, SAMPLE:KELOID FIBROBLASTS, DILUTION:1:2000
An R,Wang X,Yang L,Zhang J,Wang N,Xu F,Hou Y,Zhang H,Zhang L. Polystyrene microplastics cause granulosa cells apoptosis and fibrosis in ovary through oxidative stress in rats.Toxicology.2020 Dec 24;449:152665. doi:10.1016/j.tox.2020.152665.Epub ahead of p
Species: Rat
A00564-1 usage in article: APP:WB, SAMPLE:OVARY TISSUE, DILUTION:1:200
Li Q,Liu H,Du D,Yu Y,Ma C,Jiao F,Yao H,Lu C,Zhang W. Corrigendum: Identification of Novel Laminin- and Fibronectin-Binding Proteins by Far-Western Blot: Capturing the Adhesins of Streptococcus suis Type 2.Front Cell Infect Microbiol.2020 Oct 29;10:593413.
Species: E.coli, Human
A00564-1 usage in article: APP:WB, SAMPLE:BACTERIAL CULTURE, DILUTION:1:2000
Shi W, Bian L, Lv D, Bi S, Dai Y, Yang K, Lu H, Zhou H, Que Y, Wang D, Zhang Z, Lu N. Enhanced neural differentiation of neural stem cells by sustained release of Shh from TG2 gene-modified EMSC co-culture in vitro. Amino Acids. 2020 Nov 27. doi: 10.1007/
Species: Rat,Adenovirus
A00564-1 usage in article: APP:WB, SAMPLE:EMSCS, DILUTION:1:1000
Glyceraldehyde-3-phosphate dehydrogenase acts as an adhesin in Erysipelothrix rhusiopathiae adhesion to porcine endothelial cells and as a receptor in %u2026
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Customer Q&As
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5 Customer Q&As for Anti-Fibronectin/FN1 Antibody Picoband®
Question
We have seen staining in mouse liver. Any tips? Is anti-Fibronectin/FN1 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
From literature liver does express FN1. From Uniprot.org, FN1 is expressed in tendon, retinal pigment epithelium, aortic endothelium, amygdala, cervix, colon endothelium, endometrial tumor
rc uterine endothelium, mammary cancer, fibroblast, plasma, peripheral blood t-cell umbilical vein endothelial cell, urine, cartilage, mammary carcinoma, cervix carcinoma, liver, among other tissues. Regarding which tissues have FN1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cervix, Colon endothelium, Endometrial tumor, and Uterine endothelium, Pubmed ID: 17974005
Cartilage, Pubmed ID: 12127832
Cervix carcinoma, Pubmed ID: 17081983
Fibroblast, Pubmed ID: 2992939
Liver, Pubmed ID: 18318008, 19159218, 24275569
Mammary cancer, Pubmed ID: 3770189
Mammary carcinoma, Pubmed ID: 6304699
Peripheral blood T-cell, and Umbilical vein endothelial cell, Pubmed ID: 8406884, 3024962
Plasma, Pubmed ID: 2012601, 6630202, 14760718, 16335952
Retinal pigment epithelium, Pubmed ID: 16106752
Urine, Pubmed ID: 17614963
Boster Scientific Support
Answered: 2020-02-03
Question
We are currently using anti-Fibronectin/FN1 antibody A00564-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The anti-Fibronectin/FN1 antibody (A00564-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-27
Question
My colleagues were content with the WB result of your anti-Fibronectin/FN1 antibody. However we have seen positive staining in urine extracellular space using this antibody. Is that expected? Could you tell me where is FN1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
From literature, urine does express FN1. Generally FN1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have FN1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cervix, Colon endothelium, Endometrial tumor, and Uterine endothelium, Pubmed ID: 17974005
Cartilage, Pubmed ID: 12127832
Cervix carcinoma, Pubmed ID: 17081983
Fibroblast, Pubmed ID: 2992939
Liver, Pubmed ID: 18318008, 19159218, 24275569
Mammary cancer, Pubmed ID: 3770189
Mammary carcinoma, Pubmed ID: 6304699
Peripheral blood T-cell, and Umbilical vein endothelial cell, Pubmed ID: 8406884, 3024962
Plasma, Pubmed ID: 2012601, 6630202, 14760718, 16335952
Retinal pigment epithelium, Pubmed ID: 16106752
Urine, Pubmed ID: 17614963
Boster Scientific Support
Answered: 2019-11-19
Question
I would like using your anti-Fibronectin/FN1 antibody for extracellular matrix organization studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-05-01
Answer
We appreciate your inquiry. This A00564-1 anti-Fibronectin/FN1 antibody is validated on hela cells. It is guaranteed to work for ELISA, Flow Cytometry, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-05-01
Question
We ordered your anti-Fibronectin/FN1 antibody for WB on amygdala in the past. I am using rat, and We intend to use the antibody for ELISA next. I am looking for examining amygdala as well as peripheral blood t-cell umbilical vein endothelial cell in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2017-07-03
Answer
I took a look at the website and datasheets of our anti-Fibronectin/FN1 antibody and it appears that A00564-1 has been validated on rat in both WB and ELISA. Thus A00564-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-07-03