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Product Info Summary
| SKU: | A03260 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Claudin-5 CLDN5 Antibody
SKU/Catalog Number
A03260
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Claudin-5 CLDN5 Antibody catalog # A03260. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Claudin-5 CLDN5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03260)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Claudin 5. AA range:169-218
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03260 is reactive to CLDN5 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
23147 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03260 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of A549 cells using Claudin-5 Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using Claudin-5 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For CLDN5 (Source: Uniprot.org, NCBI)
Gene Name
CLDN5
Full Name
Claudin-5
Weight
23147 MW
Superfamily
claudin family
Alternative Names
Claudin-5;Transmembrane protein deleted in VCFS;TMDVCF;CLDN5;AWAL, TMVCF; CLDN5 AWAL, BEC1, CPETRL1, TMDVCF, TMVCF claudin 5 claudin-5|transmembrane protein deleted in VCFS|transmembrane protein deleted in velocardiofacial syndrome
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLDN5, check out the CLDN5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLDN5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Claudin-5 CLDN5 Antibody (A03260)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Claudin-5 CLDN5 Antibody
Question
Is there a BSA free version of anti-Claudin-5 antibody A03260 available?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Claudin-5 antibody A03260 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-06
Question
My colleagues were satisfied with the WB result of your anti-Claudin-5 antibody. However we have seen positive staining in brain lung tight junction. cell using this antibody. Is that expected? Could you tell me where is CLDN5 supposed to be expressed?
R. Anderson
Verified customer
Asked: 2020-04-01
Answer
Based on literature, brain lung does express CLDN5. Generally CLDN5 expresses in cell junction, tight junction. cell. Regarding which tissues have CLDN5 expression, here are a few articles citing expression in various tissues:
Brain, and Lung, Pubmed ID: 15489334
Prostate, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-04-01
Question
My question regarding product A03260, anti-Claudin-5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03260 anti-Claudin-5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-19
Question
Will A03260 anti-Claudin-5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
As indicated on the product datasheet, A03260 anti-Claudin-5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-24
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for prostate using anti-Claudin-5 antibody A03260. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-04
Question
See attached the WB image, lot number and protocol we used for prostate using anti-Claudin-5 antibody A03260. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-14
Question
We are currently using anti-Claudin-5 antibody A03260 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
The anti-Claudin-5 antibody (A03260) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-27
Question
We are interested in to test anti-Claudin-5 antibody A03260 on human prostate for research purposes, then I may be interested in using anti-Claudin-5 antibody A03260 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
The products we sell, including anti-Claudin-5 antibody A03260, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-21
Question
Will anti-Claudin-5 antibody A03260 work for WB with prostate?
B. Lewis
Verified customer
Asked: 2019-03-01
Answer
According to the expression profile of prostate, CLDN5 is highly expressed in prostate. So, it is likely that anti-Claudin-5 antibody A03260 will work for WB with prostate.
Boster Scientific Support
Answered: 2019-03-01
Question
I was wanting to use your anti-Claudin-5 antibody for WB for human prostate on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human prostate identification?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
It shows on the product datasheet, A03260 anti-Claudin-5 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human prostate in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-02
Question
I would like using your anti-Claudin-5 antibody for learning studies. Has this antibody been tested with western blotting on a549 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-05-31
Answer
We appreciate your inquiry. This A03260 anti-Claudin-5 antibody is tested on a549 cells. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-05-31
Question
I see that the anti-Claudin-5 antibody A03260 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-12-01
Question
We have seen staining in rat brain lung. Do you have any suggestions? Is anti-Claudin-5 antibody supposed to stain brain lung positively?
A. Baker
Verified customer
Asked: 2016-03-15
Answer
According to literature brain lung does express CLDN5. According to Uniprot.org, CLDN5 is expressed in right lung, prostate, brain lung, among other tissues. Regarding which tissues have CLDN5 expression, here are a few articles citing expression in various tissues:
Brain, and Lung, Pubmed ID: 15489334
Prostate, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2016-03-15
Question
Is a blocking peptide available for product anti-Claudin-5 antibody (A03260)?
S. Yang
Verified customer
Asked: 2013-10-07
Answer
We do provide the blocking peptide for product anti-Claudin-5 antibody (A03260). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-10-07
Question
Is this A03260 anti-Claudin-5 antibody reactive to the isotypes of CLDN5?
E. Carter
Verified customer
Asked: 2013-06-12
Answer
The immunogen of A03260 anti-Claudin-5 antibody is Synthesized peptide derived from the C-terminal region of human Claudin-5 at AA rangle: 140-220. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-06-12
