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Product Info Summary
| SKU: | EZ0349 |
|---|---|
| Size: | For 5 plates, 96 wells each |
| Reactive Species: | Human |
| Application: | ELISA |
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Product info
Product Name
Human Fibronectin ELISA Kit EZ-Set™ (DIY Antibody Pairs)
SKU/Catalog Number
EZ0349
Size
For 5 plates, 96 wells each
*Question: How many samples can I assay/run in this kit?
Description
Human Fibronectin ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human FN1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Fibronectin ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0349)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: from plasma
Assay Range
156 pg/ml - 10,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EZ0349 is reactive to FN1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EZ0349 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Catalog number | Description | Quantity | Storage of opened/reconstituted material |
|---|---|---|---|
| EZ0439-CA | Rabbit anti- human FN1 polyclonal antibody (Capture Antibody) | 500 µl, 0.4 mg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
| EZ0439-DA | Biotinylated goat anti- human FN1 polyclonal antibody (Detection Antibody) | 500 µl, 7 µg/mL (recommended dilution 1:100) | |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) | |
| EK0439-ST | Lyophilized recombinant human FN1 standard | 10 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Incubator.
- 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 5. Clean tubes and Eppendorf tubes.
- 6. 96 well microplate (Cat# AR1100)
- 7. Plate Sealers.
- 8. Capture Antibody Diluent: PBS.
- 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. - *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.052 | 0.091 | 0.186 | 0.317 | 0.490 | 0.890 | 1.385 | 1.990 |
Data Example Images
Click image to see more details
Human Fibronectin EZ-Set ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1.23 | 2.35 | 5.53 | 1.54 | 2.39 | 5.78 |
| Standard deviation | 0.063 | 0.099 | 0.265 | 0.12 | 0.177 | 0.399 |
| CV (%) | 5.1% | 4.2% | 4.8% | 7.8% | 7.4% | 6.9% |
Protein Target Info & Infographic
Gene/Protein Information For FN1 (Source: Uniprot.Org, NCBI)
Gene Name
FN1
Full Name
Fibronectin
Weight
272.32kDa
Alternative Names
Fibronectin; FN; Cold-insoluble globulin; CIG; Anastellin; Ugl-Y1; Ugl-Y2; Ugl-Y3; FN1; FN FN1 CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF fibronectin 1 fibronectin|cold-insoluble globulin|epididymis secretory sperm binding protein|migration-stimulating factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FN1, check out the FN1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Fibronectin ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0349)
Hello CJ!
EZ0349 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Damage-associated molecular patterns (DAMPs) released after burn are associated with inflammation and monocyte activation
Zeng R, Han M, Luo Y, Li C, Pei G, Liao W, Bai S, Ge S, Liu X, Xu G. Nephrol Dial Transplant. 2011 Apr;26(4):1149-56. Doi: 10.1093/Ndt/Gfq619. Epub 2010 Oct 19. Role Of Sema4C In Tgf-??1-Induced Mitogen-Activated Protein Kinase Activation And Epit...
Bai S, Zeng R, Zhou Q, Liao W, Zhang Y, Xu C, Han M, Pei G, Liu L, Liu X, Yao Y, Xu G. Int J Biol Sci. 2012;8(6):859-69. Doi: 10.7150/Ijbs.3490. Epub 2012 Jun 13. Cdc42-Interacting Protein-4 Promotes Tgf-??1-Induced Epithelial-Mesenchymal Transiti...
Zirkel A, Lederer M, St??hr N, Pazaitis N, H??ttelmaier S. Nucleic Acids Res. 2013 Jul;41(13):6618-36. Doi: 10.1093/Nar/Gkt410. Epub 2013 May 15. Igf2Bp1 Promotes Mesenchymal Cell Properties And Migration Of Tumor-Derived Cells By Enhancing The Ex...
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