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Key=created_at, value=2021-01-19 02:13:55
Key=updated_at, value=2024-10-25 05:03:07
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Key=name, value=Anti-Claudin 3/CLDN3 Antibody Picoband®
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Key=badge, value=free-antibody-validation
Key=price, value=370.0000
Key=weight, value=1.0000
Key=meta_title, value=Claudin 3/CLDN3 Antibody
Key=meta_description, value=Boster Bio Anti-Claudin 3/CLDN3 Antibody Picoband® catalog # A04393-5. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. Supplied as 100μg/vial in Lyophilized form antibody.
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Key=url_key, value=anti-claudin-3-cldn3-picoband-trade-antibody-a04393-5-boster
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Key=image_label, value=Figure 1. Western blot analysis of Claudin 3/CLDN3 using anti-Claudin 3/CLDN3 antibody (A04393-5).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was load
Key=small_image_label, value=Figure 1. Western blot analysis of Claudin 3/CLDN3 using anti-Claudin 3/CLDN3 antibody (A04393-5).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was load
Key=thumbnail_label, value=Figure 1. Western blot analysis of Claudin 3/CLDN3 using anti-Claudin 3/CLDN3 antibody (A04393-5).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was load
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Key=size, value=100 μg/vial
Key=gene_full_name, value=acyl-CoA synthetase long chain family member 4
Key=uniprot_id, value=O15551
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Key=sensitivity
Key=applications, value=ELISA, Flow Cytometry, IF, IHC, ICC, WB
Key=reactivity, value=Human
Key=form, value=Lyophilized
Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins.
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
Key=source_company, value=Boster A04393-5
Key=isotype, value=Rabbit IgG
Key=sample_size_available, value=30ug for $99, contact us for details
Key=related_product_skus, value=B0001-5,AR1156,AR0146
Key=product_category, value=Primary Antibodies
Key=research_category, value=Adaptive Immunity, B Cells, Hematopoietic Progenitors, Immunoglobulins, Immunology, Lymphoid, Receptors, Stem Cells
Key=clonality, value=Polyclonal
Key=swatch_image, value=/a/0/a04393-5-cldn3-primary-antibodies-wb-testing-1.jpg
Key=rating_value, value=96.6
Key=review_count, value=3
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Key=cj_related_products, value=Cystadenocarcinoma, Serous:EK1592|PB9963|EK1469^Germ Cell Migration:A06975-1|PROTP59768|A04393-1
Key=out_of_stock_until_date, value=Nov 16, 2019
Key=description, value=Boster Bio Anti-Claudin 3/CLDN3 Antibody Picoband® catalog # A04393-5. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-Claudin 3/CLDN3 Antibody Picoband® catalog # A04393-5. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-Claudin 3/CLDN3 Antibody Picoband®
Key=contents, value=Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Key=background, value=Claudin 3, also known as CLDN3, is a protein which in humans is encoded by the CLDN3 gene. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat.
Key=gene_name, value=CLDN3
Key=synonyms, value="Long-chain-fatty-acid--CoA ligase 4; Long-chain acyl-CoA synthetase 4; LACS 4; ACSL4; ACS4, FACL4, LACS4 "
Key=application_details, value=Western blot, 0.1-0.25μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Key=subcellular_localization, value= Secreted.
Key=tissue_specificity, value= Expressed in the prostate gland and prostate cancers.
Key=protein_function, value=Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed:24269233, PubMed:22633490, PubMed:21242590). Preferentially activates arachidonate and eicosapentaenoate as substrates (PubMed:21242590). Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs (By similarity). Modulates prostaglandin E2 secretion (PubMed:21242590).
Key=application_notes, value=Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=immunogen, value=E.coli-derived human Claudin 3/CLDN3 recombinant protein (Position: A101-V220).
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=23 kDa
Key=custom_attribute_2, value=WB: human MCF-7 whole cell, human Caco-2 whole cell
IHC: human renal clear cell carcinoma tissue, human gallbladder adenocarcinoma tissue, human breast invasive ductal cancer tissue, human colonic adenocarcinoma tissue, human ovarian serous adenocarcinoma tissue, human liver cancer tissue
ICC/IF: MCF-7 cell
FCM: MCF-7 cell
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