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Product Info Summary
| SKU: | A04393-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Claudin-3 Antibody
SKU/Catalog Number
A04393-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Claudin-3 Antibody catalog # A04393-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Claudin-3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04393-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Claudin 3. AA range:171-220
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04393-1 is reactive to CLDN3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
23319 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04393-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using Claudin-3 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For CLDN3 (Source: Uniprot.org, NCBI)
Gene Name
CLDN3
Full Name
Claudin-3
Weight
23319 MW
Superfamily
claudin family
Alternative Names
Claudin-3;Clostridium perfringens enterotoxin receptor 2;CPE-R 2;CPE-receptor 2;Rat ventral prostate.1 protein homolog;hRVP1;CLDN3;C7orf1, CPETR2; CLDN3 C7orf1, CPE-R2, CPETR2, HRVP1, RVP1 claudin 3 claudin-3|CPE-R 2|CPE-receptor 2|Clostridium perfringens enterotoxin receptor 2|ventral prostate.1 protein homolog|ventral prostate.1-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLDN3, check out the CLDN3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLDN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Claudin-3 Antibody (A04393-1)
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Customer Q&As
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13 Customer Q&As for Anti-Claudin-3 Antibody
Question
Will anti-Claudin-3 antibody A04393-1 work on zebrafish WB with colon?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
Our lab technicians have not tested anti-Claudin-3 antibody A04393-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Claudin-3 antibody A04393-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish colon in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-16
Question
I have a question about product A04393-1, anti-Claudin-3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04393-1 anti-Claudin-3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-20
Question
Will A04393-1 anti-Claudin-3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
As indicated on the product datasheet, A04393-1 anti-Claudin-3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-11
Question
We are currently using anti-Claudin-3 antibody A04393-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
A. Krishna
Verified customer
Asked: 2019-06-25
Answer
The anti-Claudin-3 antibody (A04393-1) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-25
Question
Is a blocking peptide available for product anti-Claudin-3 antibody (A04393-1)?
A. Walker
Verified customer
Asked: 2019-05-30
Answer
We do provide the blocking peptide for product anti-Claudin-3 antibody (A04393-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-30
Question
Is this A04393-1 anti-Claudin-3 antibody reactive to the isotypes of CLDN3?
S. Parker
Verified customer
Asked: 2019-02-26
Answer
The immunogen of A04393-1 anti-Claudin-3 antibody is Synthesized peptide derived from the C-terminal region of human Claudin-3. at AA rangle: 140-220. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-26
Question
Is there a BSA free version of anti-Claudin-3 antibody A04393-1 available?
Verified Customer
Verified customer
Asked: 2018-11-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Claudin-3 antibody A04393-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-11-05
Question
Our lab want to know about to test anti-Claudin-3 antibody A04393-1 on mouse colon for research purposes, then I may be interested in using anti-Claudin-3 antibody A04393-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Jones
Verified customer
Asked: 2017-06-26
Answer
The products we sell, including anti-Claudin-3 antibody A04393-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-26
Question
Does anti-Claudin-3 antibody A04393-1 work for WB with colon?
Verified Customer
Verified customer
Asked: 2017-05-24
Answer
According to the expression profile of colon, CLDN3 is highly expressed in colon. So, it is likely that anti-Claudin-3 antibody A04393-1 will work for WB with colon.
Boster Scientific Support
Answered: 2017-05-24
Question
I see that the anti-Claudin-3 antibody A04393-1 works with WB, what is the protocol used to produce the result images on the product page?
A. Jackson
Verified customer
Asked: 2015-03-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-03-04
Question
I was wanting to use your anti-Claudin-3 antibody for WB for mouse colon on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse colon identification?
S. Huang
Verified customer
Asked: 2014-09-01
Answer
As indicated on the product datasheet, A04393-1 anti-Claudin-3 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse colon in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-09-01
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon using anti-Claudin-3 antibody A04393-1. Let me know if you need anything else.
L. Jones
Verified customer
Asked: 2014-07-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-09
Question
Please see the WB image, lot number and protocol we used for colon using anti-Claudin-3 antibody A04393-1. Please let me know if you require anything else.
P. Jackson
Verified customer
Asked: 2013-02-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-02-07
