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Product Info Summary
| SKU: | EKC2608 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human |
| Application: | ELISA |
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Product info
Product Name
Claudin 3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2608
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The Claudin 3 (Phospho-Tyr219) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Claudin 3 (Phospho-Tyr219) protein expression profile in cells. The kit can be used for measuring the relative amounts of Claudin 3 (Phospho-Tyr219) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Claudin 3 (Phospho-Tyr219).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Claudin 3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2608)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2608 is reactive to CLDN3 in Human samples
Application Guarantee
EKC2608 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-Claudin 3 Phospho-Tyr219 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-Claudin 3 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from COLO205 cells treated with EGF 200ng/ml 30'
Protein Target Info & Infographic
Gene/Protein Information For CLDN3 (Source: Uniprot.Org, NCBI)
Gene Name
CLDN3
Full Name
Claudin-3
Weight
23319 MW
Superfamily
claudin family
Alternative Names
Claudin-3;Clostridium perfringens enterotoxin receptor 2;CPE-R 2;CPE-receptor 2;Rat ventral prostate.1 protein homolog;hRVP1;CLDN3;C7orf1, CPETR2; CLDN3 C7orf1, CPE-R2, CPETR2, HRVP1, RVP1 claudin 3 claudin-3|CPE-R 2|CPE-receptor 2|Clostridium perfringens enterotoxin receptor 2|ventral prostate.1 protein homolog|ventral prostate.1-like protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CLDN3, check out the CLDN3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CLDN3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Claudin 3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA Kit (EKC2608)
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Customer Q&As
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1 Customer Q&As for Claudin 3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA Kit
Question
Does Claudin 3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA Kit stain perineurial cells?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
Indeed, the Claudin 3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA Kit is expressed in perineurial cells.
Boster Scientific Support
Answered: 2020-01-28
