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Product Info Summary
| SKU: | A04393-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Claudin 3/CLDN3 Antibody Picoband®
SKU/Catalog Number
A04393-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Claudin 3/CLDN3 Antibody Picoband® catalog # A04393-4. Tested in IF, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Claudin 3/CLDN3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04393-4)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Claudin 3/CLDN3, which shares 73.3% and 94.1% amino acid (aa) sequence identity with human and rat Claudin 3/CLDN3, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04393-4 is reactive to Cldn3 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated molecular weight
23.319kDa
Background of Claudin-3
Claudin 3, also known as CLDN3, is a protein which in humans is encoded by the CLDN3 gene. This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is a low-affinity receptor for clostridium perfringens enterotoxin (CPE) produced by the bacterium Clostridium perfringens, and the interaction with CPE results in increased membrane permeability by forming small pores in plasma membrane. This protein is highly overexpressed in uterine carcinosarcoma. This protein is also predominantly present in brain endothelial cells, where it plays a specific role in the establishment and maintenance of blood brain barrier tight junction morphology.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04393-4 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse
Positive Control
WB: rat PC-12 whole cell
ICC/IF: NIH/3T3 cell
Validation Images & Assay Conditions
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Figure 1. IF analysis of Claudin 3/CLDN3 using anti-Claudin 3/CLDN3 antibody (A04393-4).
Claudin 3/CLDN3 was detected in immunocytochemical section of NIH/3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Claudin 3/CLDN3 Antibody (A04393-4) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 2. Western blot analysis of Claudin 3/CLDN3 using anti-Claudin 3/CLDN3 antibody (A04393-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Claudin 3/CLDN3 antigen affinity purified polyclonal antibody (Catalog # A04393-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Claudin 3/CLDN3 at approximately 23KD. The expected band size for Claudin 3/CLDN3 is at 23KD.
Protein Target Info & Infographic
Gene/Protein Information For Cldn3 (Source: Uniprot.org, NCBI)
Gene Name
Cldn3
Full Name
Claudin-3
Weight
23.319kDa
Superfamily
claudin family
Alternative Names
"Long-chain-fatty-acid--CoA ligase 4; Long-chain acyl-CoA synthetase 4; LACS 4; ACSL4; ACS4, FACL4, LACS4 " CLDN3 C7orf1, CPE-R2, CPETR2, HRVP1, RVP1 claudin 3 claudin-3|CPE-R 2|CPE-receptor 2|Clostridium perfringens enterotoxin receptor 2|ventral prostate.1 protein homolog|ventral prostate.1-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cldn3, check out the Cldn3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cldn3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Claudin 3/CLDN3 Antibody Picoband® (A04393-4)
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