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Product Info Summary
| SKU: | PB9963 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-HE4/WFDC2 Antibody Picoband®
SKU/Catalog Number
PB9963
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HE4/WFDC2 Antibody Picoband® catalog # PB9963. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HE4/WFDC2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9963)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human HE4 recombinant protein (Position: E31-F124). Human HE4 shares 40.4% and 68% amino acid (aa) sequence identity with mouse and rat HE4, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9963 is reactive to WFDC2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
25 kDa
Calculated molecular weight
12993 MW
Background of HE4/WFDC2
WAP four-disulfide core domain protein 2, also known as Human Epididymis Protein 4 (HE4), is a protein that in humans is encoded by the WFDC2 gene. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9963 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: 22RV1 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HE4 using anti-HE4 antibody (PB9963).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: 22RV1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HE4 antigen affinity purified polyclonal antibody (Catalog # PB9963) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HE4 at approximately 25 kDa. The expected band size for HE4 is at 13 kDa.
Protein Target Info & Infographic
Gene/Protein Information For WFDC2 (Source: Uniprot.org, NCBI)
Gene Name
WFDC2
Full Name
WAP four-disulfide core domain protein 2
Weight
12993 MW
Alternative Names
WAP four-disulfide core domain protein 2;Epididymal secretory protein E4;Major epididymis-specific protein E4;Putative protease inhibitor WAP5;WFDC2;HE4, WAP5; WFDC2 EDDM4, HE4, WAP5, dJ461P17.6 WAP four-disulfide core domain 2 WAP four-disulfide core domain protein 2|WAP domain containing protein HE4-V4|epididymal protein 4|epididymal secretory protein E4|epididymis secretory sperm binding protein|epididymis-specific, whey-acidic protein type, four-disulfide core|human epididymis protein 4|major epididymis-specific protein E4|putative protease inhibitor WAP5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WFDC2, check out the WFDC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WFDC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HE4/WFDC2 Antibody Picoband® (PB9963)
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No publications found for PB9963
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Customer Q&As
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1 Customer Q&As for Anti-HE4/WFDC2 Antibody Picoband®
Question
We are currently using anti-HE4/WFDC2 antibody PB9963 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
The anti-HE4/WFDC2 antibody (PB9963) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-22
