Product Info Summary
SKU: | A11252 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-CDKL1 Antibody
SKU/Catalog Number
A11252
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CDKL1 Antibody (Catalog # A11252). Tested in WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDKL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11252)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CDKL1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11252 is reactive to CDKL1 in Human, Rat
Applications
A11252 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
41671 MW
Background of CDKL1
This gene product is a member of a large family of CDC2-related serine/threonine protein kinases. It accumulates primarily in the nucleus. Two transcript variants encoding different isoforms have been found for this gene.
Meyerson M., EMBO J. 11:2909-2917(1992).
Livingston R.J., Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
Taglienti C.A., Oncogene 13:2563-2574(1996).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
![a11252 western blotting analysis 1 a11252 western blotting analysis 1](https://www.bosterbio.com/media/catalog/product/a/1/a11252-western-blotting-analysis-1.jpg)
Click image to see more details
Western blot analysis of extracts from COLO205 cells, using CDKL1 antibody A11252.
Protein Target Info & Infographic
Gene/Protein Information For CDKL1 (Source: Uniprot.org, NCBI)
Gene Name
CDKL1
Full Name
Cyclin-dependent kinase-like 1
Weight
41671 MW
Superfamily
protein kinase superfamily
Alternative Names
CDC2-related kinase 1; cyclin-dependent kinase-like 1 (CDC2-related kinase); cyclin-dependent kinase-like 1; EC 2.7.11; EC 2.7.11.22; KKIALRE; P42; Protein kinase p42 KKIALRE; serine/threonine protein kinase KKIALRE; Serine/threonine-protein kinase KKIALRE CDKL1 KKIALRE, P42 cyclin dependent kinase like 1 cyclin-dependent kinase-like 1|cyclin-dependent kinase-like 1 (CDC2-related kinase)|protein kinase p42 KKIALRE|serine/threonine protein kinase KKIALRE
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDKL1, check out the CDKL1 Infographic
![CDKL1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDKL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDKL1 Antibody (A11252)
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No publications found for A11252
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-CDKL1 Antibody
Question
Does anti-CDKL1 antibody A11252 work for WB with cerebellum?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
According to the expression profile of cerebellum, CDKL1 is highly expressed in cerebellum. So, it is likely that anti-CDKL1 antibody A11252 will work for WB with cerebellum.
Boster Scientific Support
Answered: 2020-04-13
Question
Is a blocking peptide available for product anti-CDKL1 antibody (A11252)?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
We do provide the blocking peptide for product anti-CDKL1 antibody (A11252). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-22
Question
I was wanting to use your anti-CDKL1 antibody for WB for human cerebellum on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cerebellum identification?
S. Rodriguez
Verified customer
Asked: 2019-07-23
Answer
As indicated on the product datasheet, A11252 anti-CDKL1 antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cerebellum in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-23
Question
I see that the anti-CDKL1 antibody A11252 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-20
Question
Can you help my question with product A11252, anti-CDKL1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-22
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11252 anti-CDKL1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-22
Question
We are currently using anti-CDKL1 antibody A11252 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on horse tissues as well?
T. Gonzalez
Verified customer
Asked: 2016-06-08
Answer
The anti-CDKL1 antibody (A11252) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-06-08