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Product Info Summary
| SKU: | M00248-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-CD147 (BSG) Mouse Monoclonal Antibody [Clone ID: OTI9H5]
SKU/Catalog Number
M00248-2
Size
100 µl
Description
Boster Bio Anti-BSG (CD147) mouse monoclonal antibody, clone OTI9H5 (formerly 9H5). Catalog# M00248-2. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Rat, Dog.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD147 (BSG) Mouse Monoclonal Antibody [Clone ID: OTI9H5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00248-2)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI9H5
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human BSG (NP_001719) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00248-2 is reactive to BSG in Dog, Human, Monkey, Rat
Reconstitution
Calculated molecular weight
42.2kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00248-2 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells transfected with either BSG (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BSG antibody (M00248-2)
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Anti-BSG mouse monoclonal antibody (M00248-2) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BSG.
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Immunofluorescent staining of HT29 cells using anti-BSG mouse monoclonal antibody (M00248-2).
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-BSG monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BSG monoclonal antibody.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and BSG-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-BSG monoclonal antibody M00248-2 (1:2000`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Protein Target Info & Infographic
Gene/Protein Information For BSG (Source: Uniprot.org, NCBI)
Gene Name
BSG
Full Name
Basigin
Weight
42.2kDa
Alternative Names
5F7; basigin (Ok blood group); Basigin; BSG; CD147 antigen; CD147; Collagenase stimulatory factor; EMMPRIN; EMMPRINTCSF; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK blood group antigen; OK; Tumor cell-derived collagenase stimulatory factor BSG 5F7, CD147, EMMPRIN, EMPRIN, HAb18G, OK, SLC7A11, TCSF basigin (Ok blood group) basigin|OK blood group |collagenase stimulatory factor|extracellular matrix metalloproteinase inducer|hepatoma-associated |leukocyte activation M6|tumor cell-derived collagenase stimulatory factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BSG, check out the BSG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BSG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD147 (BSG) Mouse Monoclonal Antibody [Clone ID: OTI9H5] (M00248-2)
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