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Product Info Summary
| SKU: | EK0751 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. |
Product info
Product Name
Human EMMPRIN/CD147/BSG ELISA Kit PicoKine®
SKU/Catalog Number
EK0751
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human EMMPRIN/CD147/BSG ELISA Kit PicoKine® (96 Tests). Quantitate Human BSG in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human EMMPRIN/CD147/BSG ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0751)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: T25-H205
Sensitivity
<2 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0751 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0751 is reactive to BSG in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.
Application Guarantee
EK0751 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of BSG
Basigin is a member of the immunoglobulin superfamily that is also known as EMMPRIN, short for extracellular matrix metalloproteinase inducer, and recently has been designated CD147 (cluster of differentiation 147). It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition (Miyauchi et al., 1991; Kanekura et al., 1991).This protein is a determinant for the Ok blood group system. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0751-CAP | Anti-Human BSG Pre-coated 96-well strip microplate | 1 |
| EK0751-ST | Human BSG Standard | 2 vials, 10 ng/tube |
| EK0751-DA | Human BSG Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.063 | 0.151 | 0.225 | 0.372 | 0.623 | 0.981 | 1.518 | 2.046 |
Data Example Images
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Human Emmprin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect BSG, Dilution ratio of 1:1, concentration in serum and plasma is around 340-426 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human EMMPRIN/CD147/BSG ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 69 | 232 | 1065 | 70 | 210 | 975 |
| Standard deviation | 7.64 | 16.24 | 76.68 | 5.32 | 15.54 | 77.02 |
| CV (%) | 7.6% | 7% | 7.2% | 7.6% | 7.4% | 7.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of BSG in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 69 | 69 | 64 | 65 | 66 | 2.27 | 3.4% |
| Sample 2 | 232 | 198 | 221 | 210 | 215 | 12.63 | 5.8% |
| Sample 3 | 1065 | 992 | 955 | 1022 | 1008 | 40.33 | 4% |
Protein Target Info & Infographic
Gene/Protein Information For BSG (Source: Uniprot.Org, NCBI)
Gene Name
BSG
Full Name
Basigin
Weight
42200 MW
Alternative Names
Basigin;5F7;Collagenase stimulatory factor;Extracellular matrix metalloproteinase inducer;EMMPRIN;Leukocyte activation antigen M6;OK blood group antigen;Tumor cell-derived collagenase stimulatory factor;TCSF;CD147;BSG;UNQ6505/PRO21383; BSG 5F7, CD147, EMMPRIN, EMPRIN, HAb18G, OK, SLC7A11, TCSF basigin (Ok blood group) basigin|OK blood group |collagenase stimulatory factor|extracellular matrix metalloproteinase inducer|hepatoma-associated |leukocyte activation M6|tumor cell-derived collagenase stimulatory factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on BSG, check out the BSG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BSG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human EMMPRIN/CD147/BSG ELISA Kit PicoKine® (EK0751)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human EMMPRIN/CD147/BSG ELISA Kit PicoKine®
Question
Q: how to proceed with the analysis of ELISA data? I measured Basigin level in human milk.
Verified Customer
Verified customer
Asked: 2020-10-26
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-10-26
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
H. Collins
Verified customer
Asked: 2020-05-07
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-05-07
Question
Q: can I use citrate plasma as samples in Human Basigin Picokine® ELISA Kit (Catalog # EK0751)?
G. Turner
Verified customer
Asked: 2020-02-22
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Basigin causing degradation of its protein structure. Basigin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Basigin ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-02-22
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-09-18
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Basigin in Human human milk? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
R. Kulkarni
Verified customer
Asked: 2018-12-16
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Basigin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-12-16
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-08-06
Question
Q: Can Basigin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2016-06-21
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2016-06-21
