Click image to see more details
Product Info Summary
| SKU: | M00213-7 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GFAP Rabbit Monoclonal Antibody, Clone#RM246
SKU/Catalog Number
M00213-7
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GFAP Rabbit Monoclonal Antibody, Clone#RM246 (Catalog # M00213-7). Tested in IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GFAP Rabbit Monoclonal Antibody, Clone#RM246 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00213-7)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM246
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the N-terminus of human GFAP
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human and mouse GFAP (Glial fibrillary acidic protein).
Reactive Species
M00213-7 is reactive to GFAP in Human, Mouse
Reconstitution
Calculated molecular weight
49880 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00213-7 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:200-1:500 dilution
WB: 1:1000-1:3000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of mouse brain tissue lysate, using Anti-GFAP RM246 at a 1:2500 dilution, showed GFAP (~50 kDa) expression in mouse brain.
Click image to see more details
Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue sections using Anti-GFAP RM246 at a 1:500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For GFAP (Source: Uniprot.org, NCBI)
Gene Name
GFAP
Full Name
Glial fibrillary acidic protein
Weight
49880 MW
Superfamily
intermediate filament family
Alternative Names
Glial fibrillary acidic protein;GFAP;GFAP; GFAP ALXDRD glial fibrillary acidic protein glial fibrillary acidic protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GFAP, check out the GFAP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GFAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GFAP Rabbit Monoclonal Antibody, Clone#RM246 (M00213-7)
Hello CJ!
M00213-7 has been cited in 31 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jiangong Wang,Bin Liu,Yong Xu et al. Inhibition of Histamine H3 receptor Attenuates Neuroinflammation and Cognitive Impairments in Alzheimer’s Disease via activating CREB Pathway,16 December 2020, PREPRINT (Version 1) available at Research Square [https:/
Species: Mouse
M00213-7 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:200
Ahmed S. Ahmed,JAK-1/STAT-3 pathway mediated role in aging cerebellar cortex degenerative changes of albino wistar rats, Translational Research in Anatomy, 2020,100089,ISSN 2214-854X, https://doi.org/10. 1016/j.tria.2020.100089.
Species: Rat
M00213-7 usage in article: APP:IHC, SAMPLE:CEREBELLAR CORTEX, DILUTION:1:20
Zhou Feng,Shengyan Liu,Qianwei Chen,Qiang Tan,Jishu Xian,Hua Feng,Zhi Chen,Gang Li,uPA alleviates kaolin-induced hydrocephalus by promoting the release and activation of hepatocyte growth factor in rats, Neuroscience Letters,Volume 731,2020,135011,ISSN 03
Species: Rat
M00213-7 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:200
Jian Wang,Xiaolin Li,Yu Song,Qiangfei Su,Xiakeerzhati Xiaohalati,Wen Yang,Luming Xu,Bo Cai,Guobin Wang,Zheng Wang,Lin Wang,Injectable silk sericin scaffolds with programmable shape-memory property and neuro-differentiation-promoting activity for individua
Species: Silkworm,Mouse
M00213-7 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:100
Jiangong Wang,Bin Liu,Yong Xu et al. Inhibition of Histamine H3 receptor Attenuates Neuroinflammation and Cognitive Impairments in Alzheimer’s Disease via activating CREB Pathway,16 December 2020,PREPRINT (Version 1)available at Research Square [+https://
Species: Mouse
M00213-7 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:200
Wang X,Tong Y,Zhang J,Khan N,Zhang K,Bai H,Zhang Q,Chen Y.Neuroinflammation changes with periodontal inflammation status during periodontitis in wild-type mice.Oral Dis.2020 Aug 20.doi:10.1111/odi.13618.Epub ahead of print.PMID:32815656.
Species: Mouse
M00213-7 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:50
Wang X,Zhou W,Li X,Ren J,Ji G,Du J,Tian W,Liu Q,Hao A. Graphene oxide suppresses the growth and malignancy of glioblastoma stem cell-like spheroids via epigenetic mechanisms. J Transl Med.2020 May 14;18(1):200. doi:10.1186/s12967-020-02359-z.PMID:32410622
Species: Human
M00213-7 usage in article: APP:IF, SAMPLE:GSCS, DILUTION:1:200
Deng Z,Wei Y,Yao Y,Gao S,Wang X.Let-7f promotes the differentiation of neural stem cells in rats.Am J Transl Res.2020 Sep 15;12(9):5752-5761.PMID:33042454;PMCID:PMC7540113.
Species: Rat
M00213-7 usage in article: APP:IF, SAMPLE:NSCS, DILUTION:1:100
Li X,Shi MQ,Chen C,Du JR.Phthalide derivative CD21 ameliorates ischemic brain injury in a mouse model of global cerebral ischemia: involvement of inhibition of NLRP3.Int Immunopharmacol.2020 Sep;86:106714.doi: 10.1016/j.intimp.2020.106714.Epub 2020 Jun 24
Species: Mouse
M00213-7 usage in article: APP:IF, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:100
Zhu X,Yao Y,Yang J,Zhengxie J,Li X,Hu S,Zhang A,Dong J,Zhang C,Gan G.COX-2-PGE2 signaling pathway contributes to hippocampal neuronal injury and cognitive impairment in PTZ-kindled epilepsy mice. Int Immunopharmacol. 2020 Oct;87:106801.doi: 10.1016/j.inti
Species: Mouse
M00213-7 usage in article: APP:ICC, SAMPLE:BRAIN TISSUE, DILUTION:1:100
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-GFAP Rabbit Monoclonal Antibody, Clone#RM246
Question
My question regards to test anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7 on human dorsal motor nucleus of vagus nerve for research purposes, then I may be interested in using anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
The products we sell, including anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-10
Question
Is there a BSA free version of anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7 available?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-18
Question
Our lab want to know about using your anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 for chaperone mediated autophagy studies. Has this antibody been tested with western blotting on brain tissue? We would like to see some validation images before ordering.
D. Miller
Verified customer
Asked: 2019-09-10
Answer
I appreciate your inquiry. This M00213-7 anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 is validated on mouse brain, brain tissue, tissue lysate, human brain tissue. It is guaranteed to work for IHC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-10
Question
Is this M00213-7 anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 reactive to the isotypes of GFAP?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
The immunogen of M00213-7 anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 is A peptide corresponding to the N-terminus of human GFAP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-26
Question
We purchased anti-GFAP Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
I looked at the website and datasheets of our anti-GFAP Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-07-09
Question
Our team were well pleased with the WB result of your anti-GFAP Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-03-07
Answer
Based on literature, fetal brain cortex does express GFAP. Generally GFAP expresses in cytoplasm. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2019-03-07
Question
We are currently using anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
The anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 (M00213-7) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-21
Question
I have a question about product M00213-7, anti-GFAP Rabbit Monoclonal antibody, Clone#RM246. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Taylor
Verified customer
Asked: 2018-10-23
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00213-7 anti-GFAP Rabbit Monoclonal antibody, Clone#RM246, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-23
Question
Will anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7 work for WB with dorsal motor nucleus of vagus nerve?
L. Rodriguez
Verified customer
Asked: 2018-10-11
Answer
According to the expression profile of dorsal motor nucleus of vagus nerve, GFAP is highly expressed in dorsal motor nucleus of vagus nerve. So, it is likely that anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7 will work for WB with dorsal motor nucleus of vagus nerve.
Boster Scientific Support
Answered: 2018-10-11
Question
I was wanting to use your anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 for WB for human dorsal motor nucleus of vagus nerve on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human dorsal motor nucleus of vagus nerve identification?
Verified Customer
Verified customer
Asked: 2018-03-09
Answer
It shows on the product datasheet, M00213-7 anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 has been validated for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human dorsal motor nucleus of vagus nerve in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-09
Question
I have attached the WB image, lot number and protocol we used for dorsal motor nucleus of vagus nerve using anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-03-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for dorsal motor nucleus of vagus nerve using anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7. Let me know if you need anything else.
C. Parker
Verified customer
Asked: 2018-02-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-22
Question
Is a blocking peptide available for product anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 (M00213-7)?
D. Anderson
Verified customer
Asked: 2017-09-26
Answer
We do provide the blocking peptide for product anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 (M00213-7). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-09-26
Question
Would M00213-7 anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
V. Krishna
Verified customer
Asked: 2015-09-03
Answer
As indicated on the product datasheet, M00213-7 anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-09-03
Question
We have been able to see staining in human dorsal motor nucleus of vagus nerve. Are there any suggestions? Is anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 supposed to stain dorsal motor nucleus of vagus nerve positively?
K. Anderson
Verified customer
Asked: 2014-10-06
Answer
From literature dorsal motor nucleus of vagus nerve does express GFAP. From Uniprot.org, GFAP is expressed in dorsal motor nucleus of vagus nerve, brain thalamus, brain, kidney, fetal brain cortex, fetal brain, blood, among other tissues. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2014-10-06
Question
I see that the anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7 works with WB, what is the protocol used to produce the result images on the product page?
H. Anderson
Verified customer
Asked: 2014-03-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-03-17
