Click image to see more details
Product Info Summary
| SKU: | M00209-3 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00209-3
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody catalog # M00209-3. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00209-3)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BOI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CDK1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00209-3 is reactive to CDK1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
30 kDa
Calculated molecular weight
34095 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00209-3 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of CDK1 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CDK1 (Source: Uniprot.org, NCBI)
Gene Name
CDK1
Full Name
Cyclin-dependent kinase 1
Weight
34095 MW
Superfamily
protein kinase superfamily
Alternative Names
Cyclin-dependent kinase 1;CDK1;2.7.11.22;2.7.11.23;Cell division control protein 2 homolog;Cell division protein kinase 1;p34 protein kinase;CDK1;CDC2, CDC28A, CDKN1, P34CDC2; CDK1 CDC2, CDC28A, P34CDC2 cyclin dependent kinase 1 cyclin-dependent kinase 1|cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2, G1 to S and G2 to M|cell division protein kinase 1|p34 protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK1, check out the CDK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody (M00209-3)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
15 Customer Q&As for Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-CDK1/Cdc2 Rabbit Monoclonal antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
As indicated on the product datasheet, M00209-3 anti-CDK1/Cdc2 Rabbit Monoclonal antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-13
Question
We are currently using anti-CDK1/Cdc2 Rabbit Monoclonal antibody M00209-3 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
G. Mangal
Verified customer
Asked: 2020-03-12
Answer
The anti-CDK1/Cdc2 Rabbit Monoclonal antibody (M00209-3) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-12
Question
I see that the anti-CDK1/Cdc2 Rabbit Monoclonal antibody M00209-3 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-10
Question
Is there a BSA free version of anti-CDK1/Cdc2 Rabbit Monoclonal antibody M00209-3 available?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CDK1/Cdc2 Rabbit Monoclonal antibody M00209-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-29
Question
Is this M00209-3 anti-CDK1/Cdc2 Rabbit Monoclonal antibody reactive to the isotypes of CDK1?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
The immunogen of M00209-3 anti-CDK1/Cdc2 Rabbit Monoclonal antibody is A synthesized peptide derived from human CDK1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-04
Question
We have seen staining in rat lymphoblast. Any tips? Is anti-CDK1/Cdc2 Rabbit Monoclonal antibody supposed to stain lymphoblast positively?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
According to literature lymphoblast does express CDK1. According to Uniprot.org, CDK1 is expressed in intestine, mammary cancer, skin, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-11
Question
Will anti-CDK1/Cdc2 Rabbit Monoclonal antibody M00209-3 work for WB with cervix carcinoma?
P. Dhar
Verified customer
Asked: 2019-05-02
Answer
According to the expression profile of cervix carcinoma, CDK1 is highly expressed in cervix carcinoma. So, it is likely that anti-CDK1/Cdc2 Rabbit Monoclonal antibody M00209-3 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2019-05-02
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-CDK1/Cdc2 Rabbit Monoclonal antibody M00209-3. Let me know if you need anything else.
K. Baker
Verified customer
Asked: 2019-05-02
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-02
Question
you antibody to test anti-CDK1/Cdc2 Rabbit Monoclonal antibody M00209-3 on human cervix carcinoma for research purposes, then I may be interested in using anti-CDK1/Cdc2 Rabbit Monoclonal antibody M00209-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-01-10
Answer
The products we sell, including anti-CDK1/Cdc2 Rabbit Monoclonal antibody M00209-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-10
Question
I am interested in using your anti-CDK1/Cdc2 Rabbit Monoclonal antibody for protein deubiquitination studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
J. Jha
Verified customer
Asked: 2018-11-16
Answer
I appreciate your inquiry. This M00209-3 anti-CDK1/Cdc2 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-11-16
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma using anti-CDK1/Cdc2 Rabbit Monoclonal antibody M00209-3. Please let me know if you require anything else.
G. Kulkarni
Verified customer
Asked: 2018-08-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-01
Question
Will M00209-3 anti-CDK1/Cdc2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
As indicated on the product datasheet, M00209-3 anti-CDK1/Cdc2 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-05-31
Question
Is a blocking peptide available for product anti-CDK1/Cdc2 Rabbit Monoclonal antibody (M00209-3)?
G. Wu
Verified customer
Asked: 2015-11-24
Answer
We do provide the blocking peptide for product anti-CDK1/Cdc2 Rabbit Monoclonal antibody (M00209-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-11-24
Question
I have a question about product M00209-3, anti-CDK1/Cdc2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Carter
Verified customer
Asked: 2015-07-21
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00209-3 anti-CDK1/Cdc2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-07-21
Question
Our team were satisfied with the WB result of your anti-CDK1/Cdc2 Rabbit Monoclonal antibody. However we have been able to see positive staining in intestine nucleus. cytoplasm. mitochondrion. using this antibody. Is that expected? Could you tell me where is CDK1 supposed to be expressed?
C. Evans
Verified customer
Asked: 2013-04-25
Answer
Based on literature, intestine does express CDK1. Generally CDK1 expresses in nucleus. cytoplasm. mitochondrion. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-04-25
