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Product Info Summary
| SKU: | MA1077 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Chicken, Human, Mouse |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CDK1 Antibody (Monoclonal, A17)
SKU/Catalog Number
MA1077
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CDK1 Antibody (Monoclonal, A17) catalog # MA1077. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDK1 Antibody (Monoclonal, A17) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1077)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
A17
Isotype
Mouse IgG2a
Immunogen
C-terminal two-thirds of Xenopus CDK1 expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1077 is reactive to Cdk1 in Chicken, Human, Mouse
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
18 kDa
Calculated molecular weight
34135 MW
Background of CDC2/CDK1
P34 (cdc2), also known as cell division cycle (CDC2), or cyclin-dependent kinase 1 (CDK1). CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor, that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1077 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, chicken, mouse, By Heat
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, chicken, mouse, -
Western blot, 0.5-1μg/ml, Human, chicken, mouse
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For Cdk1 (Source: Uniprot.org, NCBI)
Gene Name
Cdk1
Full Name
Cyclin-dependent kinase 1
Weight
34135 MW
Superfamily
protein kinase superfamily
Alternative Names
Cyclin-dependent kinase 1;CDK1;2.7.11.22;2.7.11.23;Cell division control protein 2 homolog;Cell division protein kinase 1;p34 protein kinase;Cdk1;Cdc2, Cdc2a, Cdkn1; CDK1 CDC2, CDC28A, P34CDC2 cyclin dependent kinase 1 cyclin-dependent kinase 1|cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2, G1 to S and G2 to M|cell division protein kinase 1|p34 protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cdk1, check out the Cdk1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cdk1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDK1 Antibody (Monoclonal, A17) (MA1077)
Hello CJ!
MA1077 has been cited in 12 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Blakemore D,Vilaplana-Lopera N,Almaghrabi R,Gonzalez E,Moya M,Ward C,Murphy G,Gambus A,Petermann E,Stewart GS,García P.MYBL2 and ATM suppress replication stress in pluripotent stem cells.EMBO Rep.2021 Mar 28:e51120.doi:10.15252/embr.202051120.Epub ahead of print.PMID:33779025.
Species: Mouse
MA1077 usage in article: APP:WB, SAMPLE:ESCS, DILUTION:NA
Wang C,Shao S,Deng L,Wang S,Zhang Y. LncRNA SNHG12 regulates the radiosensitivity of cervical cancer through the miR-148a/CDK1 pathway. Cancer Cell Int.2020 Dec 1;20(1):554.doi:10.1186/s12935-020-01654-5.PMID:33292254;PMCID:PMC7708190.
Species: Human
MA1077 usage in article: APP:WB, SAMPLE:SIHA AND HELA CELLS, DILUTION:1:500
A novel cell cycle blocker extracted from Stellera chamaejasme L. inhibits the proliferation of hepatocarcinoma cells
Human enterovirus 68 interferes with the host cell cycle to facilitate viral production
Coxsackievirus A6 induces cell cycle arrest in G0/G1 phase for viral production
Ren Y, Wang Q, Shi L, Yue W, Zhang C, Lei F. Anim Reprod Sci. 2011 Feb;123(3-4):187-91. Doi: 10.1016/J.Anireprosci.2011.01.002. Epub 2011 Jan 14. Effects Of Maternal And Dietary Selenium (Se-Enriched Yeast) On The Expression Of P34(Cdc2) And Cycli...
Yang C, Yang J, Sun M, Yan J, Meng X, Ma T. Iubmb Life. 2013 May;65(5):435-44. Doi: 10.1002/Iub.1141. Epub 2013 Feb 26. Alantolactone Inhibits Growth Of K562/Adriamycin Cells By Downregulating Bcr/Abl And P-Glycoprotein Expression.
Zeng Zj, Xiang Sg, Xue Ww, Li Hd, Ma N, Ren Zj, Xu Zj, Jiao Ch, Wang Cy, Hu Wx. Biomed Pharmacother. 2014 Sep;68(7):887-92. Doi: 10.1016/J.Biopha.2014.07.018. Epub 2014 Jul 28. The Cell Death And Dna Damages Caused By The Tet-On Regulating Hsv-Tk/...
Ye Z, Chen Z, Chen W, Xie J, Yang H, Lou Y, Yu Y. Chem Biol Interact. 2010 Jan 5;183(1):133-41. Doi: 10.1016/J.Cbi.2009.10.015. Xjw20, A Novel Oxoindole Derivative, Induces G2/M Arrest And Apoptosis Selectively In K562 Leukemia Cell Line.
Zheng Q, Liu Y, Liu W, Ma F, Zhou Y, Chen M, Chang J, Wang Y, Yang G, He G. Mol Med Rep. 2014 Jul;10(1):89-94. Doi: 10.3892/Mmr.2014.2175. Epub 2014 Apr 24. Cucurbitacin B Inhibits Growth And Induces Apoptosis Through The Jak2/Stat3 And Mapk Pathw...
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-CDK1 Antibody (Monoclonal, A17)
Question
We ordered your anti-CDK1 antibody (Monoclonal, A17) for WB on cervix carcinoma erythroleukemia last year. I am using human, and I plan to use the antibody for IHC next. Our lab want to know about examining cervix carcinoma erythroleukemia as well as leukemic t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
I have checked the website and datasheets of our anti-CDK1 antibody (Monoclonal, A17) and I see that MA1077 has been validated on human in both WB and IHC. Thus MA1077 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-18
Question
We have observed staining in mouse cervix carcinoma. What should we do? Is anti-CDK1 antibody (Monoclonal, A17) supposed to stain cervix carcinoma positively?
W. Mangal
Verified customer
Asked: 2018-11-26
Answer
Based on literature cervix carcinoma does express CDK1. Based on Uniprot.org, CDK1 is expressed in intestine, mammary cancer, skin, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-11-26
Question
Our lab were satisfied with the WB result of your anti-CDK1 antibody (Monoclonal, A17). However we have seen positive staining in mammary cancer nucleus. cytoplasm. mitochondrion. using this antibody. Is that expected? Could you tell me where is CDK1 supposed to be expressed?
R. Thomas
Verified customer
Asked: 2013-11-21
Answer
From literature, mammary cancer does express CDK1. Generally CDK1 expresses in nucleus. cytoplasm. mitochondrion. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-11-21
Question
We are currently using anti-CDK1 antibody (Monoclonal, A17) MA1077 for chicken tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says chicken, human, mouse. Is it likely that the antibody can work on feline tissues as well?
M. Miller
Verified customer
Asked: 2013-08-13
Answer
The anti-CDK1 antibody (Monoclonal, A17) (MA1077) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-08-13
