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Product Info Summary
| SKU: | MA1032 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Bovine, Human, Mouse |
| Host: | Mouse |
| Application: | ICC, WB |
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Product info
Product Name
Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1)
SKU/Catalog Number
MA1032
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1) catalog # MA1032. Tested in ICC, WB applications. This antibody reacts with Bovine, Human, Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1032)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
CY-A1
Isotype
Mouse IgG2a
Immunogen
Recombinant bovine cyclin A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1032 is reactive to Ccna2 in Bovine, Human, Mouse
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
47269 MW
Background of Cyclin A2
The mammalian A-type cyclin family consists of 2 members, cyclin A1 (CCNA1) and cyclin A2 (CCNA2). Human cyclin A gene is assigned to 4q26-q27. Cyclin A protein is synthesized and localized into the nucleus at the onset of S phase in nontransformed mammalian fibroblasts.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1032 is guaranteed for ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 1μg/ml, Human, bovine , mouse, -
Western blot, 0.5-1μg/ml, Human, bovine , mouse
Positive Control
WB: human Hela whole cell, human Jurkat whole cell
ICC: A549 cell, SMMC-7721 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cyclin A using anti-Cyclin A antibody (MA1032).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cyclin A antigen affinity purified monoclonal antibody (Catalog # MA1032) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cyclin A at approximately 55 kDa. The expected band size for Cyclin A is at 49 kDa.
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Figure 2. ICC analysis of Cyclin A using anti-Cyclin A antibody (MA1032).
Cyclin A was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml mouse anti-Cyclin A Antibody (MA1032) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. ICC analysis of Cyclin A using anti-Cyclin A antibody (MA1032).
Cyclin A was detected in an immunocytochemical section of SMMC-7721 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml mouse anti-Cyclin A Antibody (MA1032) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Ccna2 (Source: Uniprot.org, NCBI)
Gene Name
Ccna2
Full Name
Cyclin-A2
Weight
47269 MW
Superfamily
cyclin family
Alternative Names
Cyclin-A2;Cyclin-A;Ccna2;Ccna, Cyca; CCNA2 CCN1, CCNA cyclin A2 cyclin-A2|cyclin-A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ccna2, check out the Ccna2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ccna2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1) (MA1032)
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Customer Q&As
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6 Customer Q&As for Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1)
Question
Would MA1032 anti-Cyclin A antibody (Monoclonal, CY-A1) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
It shows on the product datasheet, MA1032 anti-Cyclin A antibody (Monoclonal, CY-A1) as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-06
Question
Do you have a BSA free version of anti-Cyclin A antibody (Monoclonal, CY-A1) MA1032 available?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cyclin A antibody (Monoclonal, CY-A1) MA1032 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-22
Question
Here is the WB image, lot number and protocol we used for erythroleukemia using anti-Cyclin A antibody (Monoclonal, CY-A1) MA1032. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-11
Question
Will anti-Cyclin A antibody (Monoclonal, CY-A1) MA1032 work on canine ICC with brain?
Verified Customer
Verified customer
Asked: 2019-03-07
Answer
Our lab technicians have not validated anti-Cyclin A antibody (Monoclonal, CY-A1) MA1032 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Cyclin A antibody (Monoclonal, CY-A1) MA1032 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in ICC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-07
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-Cyclin A antibody (Monoclonal, CY-A1) MA1032. Let me know if you need anything else.
T. Li
Verified customer
Asked: 2017-07-28
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-28
Question
I see that the anti-Cyclin A antibody (Monoclonal, CY-A1) MA1032 works with WB, what is the protocol used to produce the result images on the product page?
C. Mitchell
Verified customer
Asked: 2016-07-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-07-14
