Product Info Summary
SKU: | M01724-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD99 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01724-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD99 Rabbit Monoclonal Antibody catalog # M01724-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD99 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01724-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AODD-3
Isotype
Rabbit IgG
Standard Protein
A synthesized peptide derived from human CD99
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01724-1 is reactive to CD99 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
68 kDa
Calculated molecular weight
18848 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01724-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: HUVEC cell
Validation Images & Assay Conditions
![m01724 1 wb m01724 1 wb](https://www.bosterbio.com/media/catalog/product/m/0/m01724-1-wb.jpg)
Click image to see more details
Western blot analysis of CD99 expression in HUVEC cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CD99 (Source: Uniprot.org, NCBI)
Gene Name
CD99
Full Name
CD99 antigen
Weight
18848 MW
Superfamily
CD99 family
Alternative Names
12E7; antigen identified by monoclonal antibodies 12E7, F21 and O13; CD99 antigenY homolog; CD99 molecule; CD99; E2 antigen; HBA71; MIC2 (monoclonal 12E7); MIC2; MIC2Y; MSK5X; pilr-1; PILR-L; Protein MIC2; surface antigen MIC2; T-cell surface glycoprotein E2 CD99 HBA71, MIC2, MIC2X, MIC2Y, MSK5X CD99 molecule (Xg blood group) CD99 antigen|E2 antigen|MIC2 (monoclonal antibody 12E7)|T-cell surface glycoprotein E2|antigen identified by monoclonal 12E7, Y homolog|antigen identified by monoclonal antibodies 12E7, F21 and O13|cell surface antigen 12E7|cell surface antigen HBA-71|cell surface antigen O13|surface antigen MIC2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD99, check out the CD99 Infographic
![CD99 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD99: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD99 Rabbit Monoclonal Antibody (M01724-1)
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1 Customer Q&As for Anti-CD99 Rabbit Monoclonal Antibody
Question
We are currently using anti-CD99 Rabbit Monoclonal antibody M01724-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
The anti-CD99 Rabbit Monoclonal antibody (M01724-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-17