Anti-CD99 antigen CD99 Antibody

Product Name

Anti-CD99 antigen CD99 Antibody

View all CD99 Antibodies

SKU/Catalog Number

A01724-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-CD99 antigen CD99 Antibody (Catalog# A01724-1). Tested in IF, ICC, IHC-P, ELISA applications. This antibody reacts with Human, Rat, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD99 antigen CD99 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01724-1)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human CD99. Immunogen sequence location: 11-60

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01724-1 is reactive to CD99 in Human, Mouse, Rat

Applications

A01724-1 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

18848 MW

Background of CD99

Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunofluorescence, 1:200 - 1:1000
ELISA, 1:5000
Not yet tested in other applications.

Validation Images & Assay Conditions

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Immunofluorescence analysis of COS7 cells, using CD99 Antibody. The picture on the right is blocked with the synthesized peptide.

Gene/Protein Information For CD99 (Source: Uniprot.org, NCBI)

Alternative Names

12E7; antigen identified by monoclonal antibodies 12E7, F21 and O13; CD99 antigenY homolog; CD99 molecule; CD99; E2 antigen; HBA71; MIC2 (monoclonal 12E7); MIC2; MIC2Y; MSK5X; pilr-1; PILR-L; Protein MIC2; surface antigen MIC2; T-cell surface glycoprotein E2 CD99 HBA71, MIC2, MIC2X, MIC2Y, MSK5X CD99 molecule (Xg blood group) CD99 antigen|E2 antigen|MIC2 (monoclonal antibody 12E7)|T-cell surface glycoprotein E2|antigen identified by monoclonal 12E7, Y homolog|antigen identified by monoclonal antibodies 12E7, F21 and O13|cell surface antigen 12E7|cell surface antigen HBA-71|cell surface antigen O13|surface antigen MIC2

For more info on CD99, check out the CD99 Infographic

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In this infographic, you will see the following information for CD99: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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