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Product Info Summary
| SKU: | PA2179 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-FLI1 Antibody Picoband®
SKU/Catalog Number
PA2179
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FLI1 Antibody catalog # PA2179. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FLI1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2179)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human FLI1, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2179 is reactive to FLI1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
53 kDa
Calculated molecular weight
50982 MW
Background of FLI1
Friend leukemia integration 1 transcription factor, also known as Transcription factor ERGB or Proto-oncogene Fli-1 is a protein that in humans is encoded by the human FLI1 gene. The fli1 protooncogene encodes a member of the ETS family of winged helix-turn-helix transcription factors. This gene is mapped to 11q24.3. Fli-1 is activated through retroviral insertional mutagenesis in 90% of F-MuLV-induced erythroleukemias. The constitutive activation of fli-1 in erythroblasts leads to a dramatic shift in the Epo/Epo-R signal transduction pathway, blocking erythroid differentiation, activating the Ras pathway, and resulting in massive Epo-independent proliferation of erythroblasts. These results suggest that Fli-1 overexpression in erythroblasts alters their responsiveness to Epo and triggers abnormal proliferation by switching the signaling event (s) associated with terminal differentiation to proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2179 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: JURKAT Cell, RAJI Cell,
IHC: Human Tonsil Tissue, Rat Spleen Tissue
Validation Images & Assay Conditions
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Anti-FLI1 antibody, PA2179, IHC(P)
IHC(P): Human Tonsil Tissue
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Anti-FLI1 antibody, PA2179, Western blotting
Lane 1: JURKAT Cell Lysate
Lane 2: RAJI Cell Lysate
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Anti-FLI1 antibody, PA2179, IHC(P)
IHC(P): Rat Spleen Tissue
Protein Target Info & Infographic
Gene/Protein Information For FLI1 (Source: Uniprot.org, NCBI)
Gene Name
FLI1
Full Name
Friend leukemia integration 1 transcription factor
Weight
50982 MW
Superfamily
ETS family
Alternative Names
Friend leukemia integration 1 transcription factor;Proto-oncogene Fli-1;Transcription factor ERGB;FLI1; FLI1 BDPLT21, EWSR2, SIC-1 Fli-1 proto-oncogene, ETS transcription factor Friend leukemia integration 1 transcription factor|Ewing sarcoma breakpoint region 2|Friend leukemia virus integration 1|transcription factor ERGB
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FLI1, check out the FLI1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FLI1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FLI1 Antibody Picoband® (PA2179)
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No publications found for PA2179
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-FLI1 Antibody Picoband®
Question
My boss were satisfied with the WB result of your anti-FLI1 antibody. However we have seen positive staining in bone marrow nucleus. using this antibody. Is that expected? Could you tell me where is FLI1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
From what I have seen in literature, bone marrow does express FLI1. Generally FLI1 expresses in nucleus. Regarding which tissues have FLI1 expression, here are a few articles citing expression in various tissues:
Amygdala, Placenta, and Thymus, Pubmed ID: 14702039
Blood, Pubmed ID: 8439553
Bone marrow, Pubmed ID: 1522903
Leukemic T-cell, Pubmed ID: 19690332
Lymph, Pubmed ID: 15489334
Placenta, Pubmed ID: 7542907
Boster Scientific Support
Answered: 2020-05-07
Question
I have a question about product PA2179, anti-FLI1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2179 anti-FLI1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-26
Question
We have seen staining in human blood. Are there any suggestions? Is anti-FLI1 antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
Based on literature blood does express FLI1. Based on Uniprot.org, FLI1 is expressed in leukocyte, bone marrow, blood, amygdala, placenta thymus, lymph, placenta, leukemic t-cell, among other tissues. Regarding which tissues have FLI1 expression, here are a few articles citing expression in various tissues:
Amygdala, Placenta, and Thymus, Pubmed ID: 14702039
Blood, Pubmed ID: 8439553
Bone marrow, Pubmed ID: 1522903
Leukemic T-cell, Pubmed ID: 19690332
Lymph, Pubmed ID: 15489334
Placenta, Pubmed ID: 7542907
Boster Scientific Support
Answered: 2020-02-14
Question
I see that the anti-FLI1 antibody PA2179 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-12
Question
We are currently using anti-FLI1 antibody PA2179 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
The anti-FLI1 antibody (PA2179) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-31
Question
We ordered your anti-FLI1 antibody for IHC on blood in a previous project. I am using rat, and We intend to use the antibody for WB next. We want examining blood as well as bone marrow in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
I took a look at the website and datasheets of our anti-FLI1 antibody and it seems that PA2179 has been tested on rat in both IHC and WB. Thus PA2179 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-04
Question
Is a blocking peptide available for product anti-FLI1 antibody (PA2179)?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
We do provide the blocking peptide for product anti-FLI1 antibody (PA2179). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-17
Question
Does PA2179 anti-FLI1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
It shows on the product datasheet, PA2179 anti-FLI1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-23
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-FLI1 antibody PA2179. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-03
Question
Here is the WB image, lot number and protocol we used for leukocyte using anti-FLI1 antibody PA2179. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-11
Question
Does anti-FLI1 antibody PA2179 work on zebrafish WB with leukocyte?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
Our lab technicians have not tested anti-FLI1 antibody PA2179 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-FLI1 antibody PA2179 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish leukocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-14
Question
My lab would like using your anti-FLI1 antibody for dna-templated studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-08-01
Answer
I appreciate your inquiry. This PA2179 anti-FLI1 antibody is validated on human tonsil tissue, jurkat cell lysate, raji cell lysate, rat spleen tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-08-01
Question
Will anti-FLI1 antibody PA2179 work for WB with leukocyte?
Verified Customer
Verified customer
Asked: 2018-02-15
Answer
According to the expression profile of leukocyte, FLI1 is highly expressed in leukocyte. So, it is likely that anti-FLI1 antibody PA2179 will work for WB with leukocyte.
Boster Scientific Support
Answered: 2018-02-15
Question
Is this PA2179 anti-FLI1 antibody reactive to the isotypes of FLI1?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
The immunogen of PA2179 anti-FLI1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human FLI1(54-73aa QQEWINQPVRVNVKREYDHM), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-02
Question
I was wanting to use your anti-FLI1 antibody for WB for rat leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat leukocyte identification?
Verified Customer
Verified customer
Asked: 2017-06-01
Answer
You can see on the product datasheet, PA2179 anti-FLI1 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-01
Question
Is there a BSA free version of anti-FLI1 antibody PA2179 available?
Z. Miller
Verified customer
Asked: 2013-06-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FLI1 antibody PA2179 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-06-04
Question
I would like to test anti-FLI1 antibody PA2179 on rat leukocyte for research purposes, then I may be interested in using anti-FLI1 antibody PA2179 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Gonzalez
Verified customer
Asked: 2013-01-22
Answer
The products we sell, including anti-FLI1 antibody PA2179, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-01-22
