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Product Info Summary
| SKU: | M01047-2 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD79a (C-term) Rabbit Monoclonal Antibody, Clone#RM297
SKU/Catalog Number
M01047-2
Size
100uL
Form
Liquid
Description
Boster Bio Anti-CD79a (C-term) Rabbit Monoclonal Antibody, Clone#RM297 (Catalog # M01047-2). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD79a (C-term) Rabbit Monoclonal Antibody, Clone#RM297 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01047-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM297
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human CD79a
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human CD79a
Reactive Species
M01047-2 is reactive to CD79A in Human
Reconstitution
Calculated molecular weight
25038 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01047-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:100-1:200 dilution
WB: 1:100-1:200 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of Raji cells lysate using anti-CD79a rabbit monoclonal antibody (Clone RM297) at a 1:200 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-CD79a rabbit monoclonal antibody (Clone RM297) at a 1:200 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CD79A (Source: Uniprot.org, NCBI)
Gene Name
CD79A
Full Name
B-cell antigen receptor complex-associated protein alpha chain
Weight
25038 MW
Alternative Names
B-cell antigen receptor complex-associated protein alpha chain;Ig-alpha;MB-1 membrane glycoprotein;Membrane-bound immunoglobulin-associated protein;Surface IgM-associated protein;CD79a;CD79A;IGA, MB1; CD79A IGA, MB-1 CD79a molecule B-cell receptor complex-associated protein alpha chain|CD79a (immunoglobulin-associated alpha)|CD79a molecule, immunoglobulin-associated alpha|MB-1 membrane glycoprotein|ig-alpha|membrane-bound immunoglobulin-associated protein|surface IgM-associated protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD79A, check out the CD79A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD79A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD79a (C-term) Rabbit Monoclonal Antibody, Clone#RM297 (M01047-2)
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No publications found for M01047-2
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CD79a (C-term) Rabbit Monoclonal Antibody, Clone#RM297
Question
Would M01047-2 anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
It shows on the product datasheet, M01047-2 anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-26
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tonsil using anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 M01047-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-21
Question
I am interested in to test anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 M01047-2 on human tonsil for research purposes, then I may be interested in using anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 M01047-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
The products we sell, including anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 M01047-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-09
Question
Is a blocking peptide available for product anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 (M01047-2)?
S. Anderson
Verified customer
Asked: 2019-08-07
Answer
We do provide the blocking peptide for product anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 (M01047-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-07
Question
I see that the anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 M01047-2 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-27
Question
Would anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 M01047-2 work for IHC with tonsil?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
According to the expression profile of tonsil, CD79A is highly expressed in tonsil. So, it is likely that anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 M01047-2 will work for IHC with tonsil.
Boster Scientific Support
Answered: 2019-05-31
Question
My question regarding product M01047-2, anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01047-2 anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-04-26
Question
I have attached the WB image, lot number and protocol we used for tonsil using anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 M01047-2. Please let me know if you require anything else.
M. Evans
Verified customer
Asked: 2019-03-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-26
Question
I am interested in using your anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 for b cell proliferation studies. Has this antibody been tested with western blotting on raji cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-08-17
Answer
I appreciate your inquiry. This M01047-2 anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 is tested on raji cells, human tonsil tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-08-17
Question
Is this M01047-2 anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 reactive to the isotypes of CD79A?
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
The immunogen of M01047-2 anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 is A peptide corresponding to the C-terminus of human CD79a. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-20
Question
I was wanting to use your anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 for IHC for human tonsil on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human tonsil identification?
Verified Customer
Verified customer
Asked: 2017-08-09
Answer
As indicated on the product datasheet, M01047-2 anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human tonsil in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-09
Question
We are currently using anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 M01047-2 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
S. Wu
Verified customer
Asked: 2017-03-03
Answer
The anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 (M01047-2) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-03-03
Question
We ordered your anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone
C. Walker
Verified customer
Asked: 2015-05-28
Answer
I took a look at the website and datasheets of our anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2015-05-28
Question
My colleagues were happy with the WB result of your anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone
S. Kulkarni
Verified customer
Asked: 2015-05-20
Answer
According to literature, small intestine does express CD79A. Generally CD79A expresses in cell membrane. Regarding which tissues have CD79A expression, here are a few articles citing expression in various tissues:
Small intestine, Pubmed ID: 7514267
Tonsil, Pubmed ID: 1534761
Boster Scientific Support
Answered: 2015-05-20
Question
We have been able to see staining in human small intestine. Are there any suggestions? Is anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 supposed to stain small intestine positively?
A. Mangal
Verified customer
Asked: 2015-02-05
Answer
According to literature small intestine does express CD79A. According to Uniprot.org, CD79A is expressed in adrenal gland, tonsil, small intestine, among other tissues. Regarding which tissues have CD79A expression, here are a few articles citing expression in various tissues:
Small intestine, Pubmed ID: 7514267
Tonsil, Pubmed ID: 1534761
Boster Scientific Support
Answered: 2015-02-05
Question
Is there a BSA free version of anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 M01047-2 available?
D. Bhatt
Verified customer
Asked: 2013-05-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD79a (C-term) Rabbit Monoclonal antibody, Clone#RM297 M01047-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-05-09
