Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | PB9168 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD79a Antibody Picoband®
SKU/Catalog Number
PB9168
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD79a Antibody Picoband® catalog # PB9168. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD79a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9168)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD79a recombinant protein (Position: T121-P226). Human CD79a shares 91% amino acid (aa) sequence identity with mouse CD79a.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9168 is reactive to CD79A in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
44 kDa
Calculated molecular weight
25038 MW
Background of CD79A
Cluster of differentiation CD79A also known as B-cell antigen receptor complex-associated protein alpha chain and MB-1 membrane glycoprotein, is a protein that in humans is encoded by the CD79A gene. It is mapped to 19q13.2. CD79A is a membrane protein with an extracellular immunoglobulin domain, a single span transmembrane region and a short cytoplasmic domain. Genetic deletion of the transmembrane exon of CD79A results in loss of CD79A protein and a complete block of B cell development at the pro to pre B cell transition. Similarly, humans with homozygous splice variants in CD79A predicted to result in loss of the transmembrane region and a truncated or absent protein display agammaglobulinemia and no peripheral B cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9168 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human Daudi whole cell, human Ramos whole cell
IHC: human tonsil tissue
IF: human tonsil tissue
FCM: Jurkat cell, Ramos cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD79A using anti-CD79A antibody (PB9387).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human Ramos whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD79A antigen affinity purified polyclonal antibody (Catalog # PB9387) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD79A at approximately 44 kDa. The expected band size for CD79A is at 25 kDa.
Click image to see more details
Figure 2. IHC analysis of CD79A using anti-CD79A antibody (PB9168).
CD79A was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD79A Antibody (PB9168) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. Flow Cytometry analysis of Jurkat cells using anti-CD79A antibody (PB9168).
Overlay histogram showing Jurkat cells stained with PB9168 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD79A Antibody (PB9168, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 3. IF analysis of CD79A using anti-CD79A antibody (PB9168).
CD79A was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD79A Antibody (PB9168) overnight at 4°C. DyLight®550 conjugated goat anti-rabbit IgG (BA1135) was used as secondary antibody and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. Flow Cytometry analysis of Ramos cells using anti-CD79A antibody (PB9168).
Overlay histogram showing Ramos cells stained with PB9168 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD79A Antibody (PB9168, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CD79A (Source: Uniprot.org, NCBI)
Gene Name
CD79A
Full Name
B-cell antigen receptor complex-associated protein alpha chain
Weight
25038 MW
Alternative Names
B-cell antigen receptor complex-associated protein alpha chain;Ig-alpha;MB-1 membrane glycoprotein;Membrane-bound immunoglobulin-associated protein;Surface IgM-associated protein;CD79a;CD79A;IGA, MB1; CD79A IGA, MB-1 CD79a molecule B-cell receptor complex-associated protein alpha chain|CD79a (immunoglobulin-associated alpha)|CD79a molecule, immunoglobulin-associated alpha|MB-1 membrane glycoprotein|ig-alpha|membrane-bound immunoglobulin-associated protein|surface IgM-associated protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD79A, check out the CD79A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD79A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD79a Antibody Picoband® (PB9168)
Hello CJ!
No publications found for PB9168
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD79a Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CD79a Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-CD79a Antibody Picoband®
Question
I am looking for using your anti-CD79a antibody for adaptive immune response studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
G. Miller
Verified customer
Asked: 2020-01-30
Answer
We appreciate your inquiry. This PB9168 anti-CD79a antibody is validated on human tonsil tissue, placenta tissue, tissue lysate, rat spleen tissue. It is guaranteed to work for IHC-P, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-30
Question
My boss were satisfied with the WB result of your anti-CD79a antibody. However we have observed positive staining in tonsil cell membrane using this antibody. Is that expected? Could you tell me where is CD79A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
From what I have seen in literature, tonsil does express CD79A. Generally CD79A expresses in cell membrane. Regarding which tissues have CD79A expression, here are a few articles citing expression in various tissues:
Small intestine, Pubmed ID: 7514267
Tonsil, Pubmed ID: 1534761
Boster Scientific Support
Answered: 2019-10-07
Question
We have observed staining in human small intestine. Any tips? Is anti-CD79a antibody supposed to stain small intestine positively?
T. Miller
Verified customer
Asked: 2019-04-09
Answer
Based on literature small intestine does express CD79A. Based on Uniprot.org, CD79A is expressed in adrenal gland, tonsil, small intestine, among other tissues. Regarding which tissues have CD79A expression, here are a few articles citing expression in various tissues:
Small intestine, Pubmed ID: 7514267
Tonsil, Pubmed ID: 1534761
Boster Scientific Support
Answered: 2019-04-09
Question
We are currently using anti-CD79a antibody PB9168 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on goat tissues as well?
C. Li
Verified customer
Asked: 2013-10-11
Answer
The anti-CD79a antibody (PB9168) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-10-11
Question
We ordered your anti-CD79a antibody for IHC-P on small intestine in a previous experiment. I am using human, and I plan to use the antibody for WB next. I am looking for examining small intestine as well as adrenal gland in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
P. Johnson
Verified customer
Asked: 2013-08-27
Answer
I looked at the website and datasheets of our anti-CD79a antibody and it appears that PB9168 has been validated on human in both IHC-P and WB. Thus PB9168 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-08-27
