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Product Info Summary
| SKU: | PA2007 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-TRAF2 Antibody Picoband®
SKU/Catalog Number
PA2007
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRAF2 Antibody catalog # PA2007. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRAF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2007)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human TRAF2, different from the related rat and mouse sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2007 is reactive to TRAF2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
56 kDa
Calculated molecular weight
55859 MW
Background of TRAF-2
TRAF2 (TNF Receptor-Associated Factor 2), also called TRAP, is a protein that in humans is encoded by the TRAF2 gene. The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF2 is a common signal transducer for TNFR2 and CD40 that mediates activation of NF-kappa-B. Rothe et al. (1996) identified ITRAF, which binds to TRAF1, TRAF2, and TRAF3, and that when overexpressed inhibits TRAF2-mediated NF-kappa-B activation. They proposed that ITRAF is an inhibitor of TRAF function that regulates TRAF protein activity by sequestering TRAFs in a latent state in the cytoplasm. Kanamori et al. (2002) found that mouse Traf2 interacted directly with T2bp, and they presented evidence that T2BP is involved in TNF-mediated signaling by its interaction with TRAF2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2007 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: human Raji whole cell, rat PC-12 whole cell, mouse spleen tissue
IHC: Human Tonsil Tissue
ICC: HELA Cell
Validation Images & Assay Conditions
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Anti-TRAF2 antibody, PA2007, IHC(P)
IHC(P): Human Tonsil Tissue
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Anti-TRAF2 antibody, PA2007, ICC
ICC: HELA Cell
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Figure 1. Western blot analysis of TRAF2 using anti-TRAF2 antibody (PA2007).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysate,
Lane 2: rat PC-12 whole cell lysate,
Lane 3: mouse spleen tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF2 antigen affinity purified polyclonal antibody (Catalog # PA2007) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF2 at approximately 56KD. The expected band size for TRAF2 is at 56KD.
Protein Target Info & Infographic
Gene/Protein Information For TRAF2 (Source: Uniprot.org, NCBI)
Gene Name
TRAF2
Full Name
TNF receptor-associated factor 2
Weight
55859 MW
Superfamily
TNF receptor-associated factor family
Alternative Names
TNF receptor-associated factor 2;6.3.2.-;E3 ubiquitin-protein ligase TRAF2;Tumor necrosis factor type 2 receptor-associated protein 3;TRAF2;TRAP3; TRAF2 MGC:45012, RNF117, TRAP, TRAP3 TNF receptor associated factor 2 TNF receptor-associated factor 2|E3 ubiquitin-protein ligase TRAF2|RING-type E3 ubiquitin transferase TRAF2|tumor necrosis factor type 2 receptor associated protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRAF2, check out the TRAF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRAF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRAF2 Antibody Picoband® (PA2007)
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Customer Q&As
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6 Customer Q&As for Anti-TRAF2 Antibody Picoband®
Question
Would anti-TRAF2 antibody PA2007 work for WB with stomach uterus?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
According to the expression profile of stomach uterus, TRAF2 is highly expressed in stomach uterus. So, it is likely that anti-TRAF2 antibody PA2007 will work for WB with stomach uterus.
Boster Scientific Support
Answered: 2019-08-30
Question
Will anti-TRAF2 antibody PA2007 work on bovine IHC with brain?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
Our lab technicians have not validated anti-TRAF2 antibody PA2007 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-TRAF2 antibody PA2007 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-23
Question
I see that the anti-TRAF2 antibody PA2007 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-31
Question
Is a blocking peptide available for product anti-TRAF2 antibody (PA2007)?
Verified Customer
Verified customer
Asked: 2019-02-05
Answer
We do provide the blocking peptide for product anti-TRAF2 antibody (PA2007). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-05
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for stomach uterus using anti-TRAF2 antibody PA2007. Let me know if you need anything else.
J. Collins
Verified customer
Asked: 2018-12-21
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-21
Question
I have a question about product PA2007, anti-TRAF2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Bhatt
Verified customer
Asked: 2014-03-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2007 anti-TRAF2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-03-17
